An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages
文献类型: 外文期刊
第一作者: Ma, Jian
作者: Ma, Jian;Wang, Shan-Shan;Lin, Yue-Zhi;Liu, Hai-Fang;Wei, Hua-Mian;Du, Cheng;Wang, Xue-Feng;Zhou, Jian-Hua
作者机构:
关键词: EIAV;TLRs;Type I IFNs;Protective immunity
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN: 0378-1135
年卷期: 2013 年 166 卷 1-2 期
页码:
收录情况: SCI
摘要: Activations of endosomal TLRs include TLR3, TLR7/8, and TLR9 stimulates the production of cytokines, such as type I interferons (IFNs), and therefore involves in virus-host interactions. In the present study, two equine anemia virus (EIAV) strains EIAV(FDDV13) and EIAV(FDDV)3-8, which showed different induction on protective immunity, were compared regarding their ability to regulate the expression of endosomal TLRs, as well as type I IFNs, after infection of equine monocyte-derived macrophages (eMDMs). Our results showed that EIAV(FDDV13) dramatically up-regulated the expression of TLR3 and IFN beta and less robustly up-regulated the expression of TRL9 and IFN alpha 1, whereas EIAV(FDDV)3-8 induced significantly lower expression of type I IFN mRNA and protein and more strongly down-regulated the expression of TLR7 and TLR8. In addition, no significant differences in cell apoptosis were observed between these two strains. Given that the genomic variation of EIAV(FDDV)13 is considerably higher than that of molecular clone EIAV(FDDV)3-8, our results suggest that stronger TLR3 activation and increased INF beta production induced by the multi-species strain are associated with an effective vaccine-elicited protective immune response. (C) 2013 Elsevier B.V. All rights reserved.
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