An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages

文献类型: 外文期刊

第一作者: Ma, Jian

作者: Ma, Jian;Wang, Shan-Shan;Lin, Yue-Zhi;Liu, Hai-Fang;Wei, Hua-Mian;Du, Cheng;Wang, Xue-Feng;Zhou, Jian-Hua

作者机构:

关键词: EIAV;TLRs;Type I IFNs;Protective immunity

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN: 0378-1135

年卷期: 2013 年 166 卷 1-2 期

页码:

收录情况: SCI

摘要: Activations of endosomal TLRs include TLR3, TLR7/8, and TLR9 stimulates the production of cytokines, such as type I interferons (IFNs), and therefore involves in virus-host interactions. In the present study, two equine anemia virus (EIAV) strains EIAV(FDDV13) and EIAV(FDDV)3-8, which showed different induction on protective immunity, were compared regarding their ability to regulate the expression of endosomal TLRs, as well as type I IFNs, after infection of equine monocyte-derived macrophages (eMDMs). Our results showed that EIAV(FDDV13) dramatically up-regulated the expression of TLR3 and IFN beta and less robustly up-regulated the expression of TRL9 and IFN alpha 1, whereas EIAV(FDDV)3-8 induced significantly lower expression of type I IFN mRNA and protein and more strongly down-regulated the expression of TLR7 and TLR8. In addition, no significant differences in cell apoptosis were observed between these two strains. Given that the genomic variation of EIAV(FDDV)13 is considerably higher than that of molecular clone EIAV(FDDV)3-8, our results suggest that stronger TLR3 activation and increased INF beta production induced by the multi-species strain are associated with an effective vaccine-elicited protective immune response. (C) 2013 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]The effects of H3N2 swine influenza virus infection on TLRs and RLRs signaling pathways in porcine alveolar macrophages. Zhang, Jinqiu,Hou, Jibo,Zhang, Jinqiu,Miao, Jinfeng,Lu, Chengping. 2015

[2]Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV(FDDV13). Qi, Xu,Su, Zhiqiang,Wang, Xuefeng,Wang, Shuai,Lin, Yuezhi,Jiang, Chenggang,Ma, Jian,Zhao, Liping,Lv, Xiaoling,Shen, Rongxian,Kong, Xiangang,Zhou, Jianhua,Wang, Xuefeng,Wang, Fenglong,Ma, Jian. 2010

[3]A pilot study on interaction between donkey tetherin and EIAV stains with different virulent and replication characteristics. Yao, Qiucheng,Li, Yanfei,Yao, Qiucheng,Ma, Jian,Wang, Xuefeng,Guo, Miaomiao,Wang, Xiaojun.

[4]A proviral derivative from a reference attenuated EIAV vaccine strain failed to elicit protective immunity. Ma, Jian,Shi, Nan,Jiang, Cheng-Gang,Lin, Yue-Zhi,Wang, Xue-Feng,Wang, Shuai,Lv, Xiao-Ling,Zhao, Li-Ping,Kong, Xian-Gang,Zhou, Jian-Hua,Shen, Rong-Xian,Ma, Jian,Shao, Yi-Ming. 2011

[5]Antiviral potency and functional analysis of tetherin orthologues encoded by horse and donkey. Yin, Xin,Gu, Qinyong,Wei, Ping,Yin, Xin,Guo, Miaomiao,Gu, Qinyong,Wu, Xingliang,Wang, Xiaojun. 2014

[6]Development and Application of an Indirect ELISA for the Detection of gp45 Antibodies to Equine Infectious Anemia Virus. Du, Cheng,Li, Yi-Jing,Du, Cheng,Hu, Zhe,Hu, Sen-Dong,Lin, Yue-Zhi,Wang, Xiaojun. 2018

[7]Mice transgenic for equine cyclin T1 and ELR1 are susceptible to equine infectious anemia virus infection. Du, Cheng,Ma, Jian,Liu, Qiang,Li, Yun-Fei,He, Xi-Jun,Lin, Yue-Zhi,Wang, Xue-Feng,Meng, Qing-Wen,Wang, Xiaojun,Zhou, Jian-Hua,Du, Cheng. 2015

[8]Structural and biochemical insights into the V/I505T mutation found in the EIAV gp45 vaccine strain. Du, Jiansen,Ma, Jing,Wang, Jianxin,Liu, Fang,Qiao, Wentao,Liu, Xinqi,Wang, Xuefeng,Qin, Yuyin,Zhu, Chunhui,Zhou, Jianhua,Shao, Yiming,Shao, Yiming. 2014

[9]The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages. Lin, Yue-Zhi,Cao, Xue-Zhi,Li, Liang,Li, Li,Jiang, Cheng-Gang,Wang, Xue-Feng,Ma, Jian,Zhou, Jian-Hua. 2011

[10]Genetic Evolution during the development of an attenuated EIAV vaccine. Wang, Xue-Feng,Lin, Yue-Zhi,Li, Qiang,Liu, Qiang,Zhao, Wei-Wei,Du, Cheng,Chen, Jie,Wang, Xiaojun,Zhou, Jian-Hua,Wang, Xue-Feng,Li, Qiang,Zhou, Jian-Hua. 2016

[11]Reverse mutation of the virulence-associated S2 gene does not cause an attenuated equine infectious anemia virus strain to revert to pathogenicity. Gao, Xu,Jiang, Cheng-Gang,Wang, Xue-Feng,Lin, Yue-Zhi,Ma, Jian,Han, Xiu-E,Zhao, Li-Ping,Shen, Rong-Xian,Xiang, Wen-Hua,Zhou, Jian-Hua,Gao, Xu,Han, Xiu-E.

[12]Proteomic alteration of equine monocyte-derived macrophages infected with equine infectious anemia virus. Du, Cheng,Liu, Hai-Fang,Lin, Yue-Zhi,Wang, Xue-Feng,Ma, Jian,Wang, Xiaojun,Zhou, Jian-Hua,Du, Cheng,Li, Yi-Jing,Zhou, Jian-Hua.

[13]A Unique Evolution of the S2 Gene of Equine Infectious Anemia Virus in Hosts Correlated with Particular Infection Statuses. Wang, Xue-Feng,Wang, Shuai,Liu, Qiang,Lin, Yue-Zhi,Du, Cheng,Tang, Yan-Dong,Na, Lei,Wang, Xiaojun,Zhou, Jian-Hua,Zhou, Jian-Hua. 2014

[14]Genetic variation in the long terminal repeat associated with the transition of Chinese equine infectious anemia virus from virulence to avirulence. Wei, Lili,Lu, Xiaoling,Zhao, Liping,Xiang, Wenhua,Xue, Fei,Shen, Rongxian,Wang, Xiaojun,Wei, Lili,Fan, Xiujuan,Zhang, Xiaoyan,Shao, Yiming. 2009

[15]An attenuated EIAV vaccine strain induces significantly different immune responses from its pathogenic parental strain although with similar in vivo replication pattern. Lin, Yue-Zhi,Shen, Rong-Xian,Zhu, Zhen-Ying,Deng, Xi-Lin,Cao, Xue-Zhi,Wang, Xue-Feng,Ma, Jian,Jiang, Cheng-Gang,Zhao, Li-Ping,Lv, Xiao-Ling,Zhou, Jian-Hua,Shao, Yi-Ming. 2011

[16]The integration of a macrophage-adapted live vaccine strain of equine infectious anaemia virus (EIAV) in the horse genome. Liu, Qiang,Wang, Xue-Feng,Du, Cheng,Lin, Yue-Zhi,Ma, Jian,Zhou, Jian-Hua,Wang, Xiaojun,Wang, Yu-Hong. 2017

[17]C-terminal truncation of the transmembrane protein of an attenuated lentiviral vaccine alters its in vitro but not in vivo replication and weakens its potential pathogenicity. Jiang, Cheng-Gang,Gao, Xu,Ma, Jian,Lin, Yue-Zhi,Wang, Xue-Feng,Zhao, Li-Ping,Zhou, Jian-Hua,Jiang, Cheng-Gang,Liu, Di,Hua, Yue-Ping,Gao, Xu. 2011

[18]Double-stranded RNA-specific adenosine deaminase 1 (ADAR1) promotes EIAV replication and infectivity. Tang, Yan-Dong,Na, Lei,Fu, Li-Hua,Yang, Fei,Zhu, Chun-Hui,Tang, Li,Wang, Jia-Yi,Li, Zhan,Wang, Xue-Feng,Wang, Xiaojun,Zhou, Jian-Hua,Tang, Yan-Dong,Li, Cheng-Yao,Li, Qiang,Zhou, Jian-Hua.

[19]Amino acid mutations of the infectious clone from Chinese EIAV attenuated vaccine resulted in reversion of virulence. Shen, T,Liang, H,Tong, X,Fan, XJ,He, X,Ma, Y,Xiang, WH,Shen, RX,Zhang, XY,Shao, YM. 2006

[20]Identification and characterization of a common B-cell epitope on EIAV capsid proteins. Hu, Zhe,Chang, Hao,Chu, Xiaoyu,Li, Shuang,Wang, Meiyue,Wang, Xiaojun.

作者其他论文 更多>>