Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP)
文献类型: 外文期刊
第一作者: Qiao, Jun
作者: Qiao, Jun;Wang, Junwei;Meng, Qingling;Wang, Guochao;Liu, Yucheng;He, Zhihao;Yang, Haibo;Zhang, Zaichao;Chen, Chuangfu;Meng, Qingling;Cai, Xuepeng
作者机构:
关键词: Rapid detection;Akabane virus;Reverse transcription loop-mediated isothermal amplification assay
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2013 年 10 卷
页码:
收录情况: SCI
摘要: Background: Akabane disease, caused by Akabane virus, is an insect-transmitted disease of ruminants that is primarily characterized by fetal damage. Methods and results: In this study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Akabane virus was successfully developed. The primers were designed to target the highly conserved fragment of nucleoprotein from the Akabane virus. The results indicate that the assay is highly specific and sensitive with a detection limit of 5.0 TCID50(/)mL within a 60-min incubation time. A total of 126 abortive samples collected from Xinjiang province were detected by the established RT-LAMP. The results of RT-LAMP assay showed 96.8% agreement with the semi-nested RT-PCR. Conclusion: This study is to first to develop a rapid, sensitive, and accurate method for the detection of Akabane virus, which may be used to screen clinical samples in developing countries or regions.
分类号:
- 相关文献
作者其他论文 更多>>
-
2023 International African Swine Fever Workshop: Critical Issues That Need to Be Addressed for ASF Control
作者:Wang, Lihua;Li, Yuzhen;Shi, Jishu;Ganges, Llilianne;Ganges, Llilianne;Dixon, Linda K.;Netherton, Christopher L.;Reis, Ana L.;Bu, Zhigao;Zhao, Dongming;Quang Lam Truong;Richt, Juergen A.;Jin, Meilin;Peng, Guiqing;Benarafa, Charaf;Summerfield, Artur;Benarafa, Charaf;Summerfield, Artur;Benarafa, Charaf;Summerfield, Artur;Weng, Changjiang;Han, Jun;Penrith, Mary-Louise;Mo, Yupeng;Su, Zhipeng;Dang Vu Hoang;Pogranichniy, Roman M.;Balaban-Oglan, David-Adrian;Balaban-Oglan, David-Adrian;Wang, Kewen;Cai, Xuepeng
关键词:African swine fever; workshop; platform; highlights; lessons; control; prevention
-
Blue Light Attracts More Spodoptera frugiperda Moths and Promotes Their Flight Speed
作者:Yang, Haibo;Lu, Jing;Zhu, Pinhong;Sun, Yalan;Hu, Zhenjie;Li, Dingxu;Huang, Jianrong
关键词:Spodoptera frugiperda; sensitive wavelength; flight capability; light trap; light pollution
-
Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation
作者:Cao, Junfeng;Rahman, Saeed ur;Qin, Wei;Wang, Yuliang;Tang, Kexuan;Chen, Zhiwen;Liu, Zehui;Qiao, Jun;Zhao, Jianguo;Chen, Zhiwen;Lin, Jialing;Hou, Lipan;Huang, Chaochen;Wen, Tingting;Li, Chenyi;Wang, Luyao;Zhao, Yongyan;Wang, Luyao;Zhao, Yongyan;Yan, Ning;Wang, Jie;Si, Tong;Shi, Yannan
关键词:Artemisia annua; artemisinin; glandular secreting trichomes GSTs; miRNA; graphene
-
STnc1280, a trans- coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium
作者:Ning, Chengcheng;Li, Na;Wang, Lixia;Guo, Yun;Ji, Chunhui;Li, Zhiyuan;Shang, Yunxia;Sun, Yaoqiang;Huang, Xiaoxing;Leng, Qingwen;Meng, Qingling;Qiao, Jun;Ning, Chengcheng;Zhang, Xingxing;Cai, Xuepeng
关键词:modulation; Salmonella Typhimurium; sRNA; STnc1280; virulence
-
Development of polymerase chain reaction-lateral flow dipstick assay for detection of Mycoplasma bovis in cattle
作者:Song, Shengnan;Guo, Jia;Zhao, Yang;Wang, Yong;Wang, Zhen;Chen, Chuangfu;Shi, Feng;Zhang, Qian
关键词:Mycoplasma bovis; Biotin-labeled oligonucleotides; Colloidal gold-based; Polymerase chain reaction-lateral flow dipstick assay; Nucleic acid; Visual detection
-
The MADS-box transcription factor GmFULc promotes GmZTL4 gene transcription to modulate maturity in soybean
作者:Sun, Jingzhe;Fan, Yuhuan;Ma, Xiaofei;Ji, Yujia;Liu, Gaoyuan;Zhang, Xiaoming;Zhao, Lin;Sun, Jingzhe;Liu, Yucheng;Tian, Zhixi;Zheng, Yuhong;Wang, Shuming;Xue, Yongguo;Li, Yang
关键词:flowering time; GmFULc; GmZTL4; maturity; photoperiod
-
Duplication and sub-functionalization of flavonoid biosynthesis genes plays important role in Leguminosae root nodule symbiosis evolution
作者:Liu, Tengfei;Liu, Zhi;Yuan, Yaqin;Fan, Jingwei;Xiang, Shuaiying;Yang, Xia;Liu, Yucheng;Liu, Shulin;Zhang, Min;Shen, Yanting;Tian, Zhixi;Liu, Tengfei;Liu, Haiyue;Liu, Zhi;Yuan, Yaqin;Xiang, Shuaiying;Yang, Xia;Jiao, Yuannian;Xie, Fang;Li, Jiayang;Tian, Zhixi;Liu, Haiyue;Xie, Fang;Xian, Wenfei;Cheng, Shifeng;Xian, Wenfei;Liu, Zhi;Jiao, Yuannian;Doyle, Jeff J.;Li, Jiayang;Li, Jiayang;Li, Jiayang
关键词:CHI1; duplication; gene gain; Leguminosae; root nodule symbiosis evolution; sub-functionalization