A sensitive chemiluminescent enzyme immunoassay for carbofuran residue in vegetable, fruit and environmental samples

文献类型： 外文期刊

第一作者： Jin, Maojun

作者： Jin, Maojun;Shao, Hua;Jin, Fen;Wang, Jing;Zhu, Guonian;Liu, Shaoying;Guo, Yirong;Jin, Renyao

作者机构：

关键词： chemiluminescent enzyme immunoassay (CLEIA);assay optimisation;carbofuran;ultra performance liquid chromatography (UPLC)-MS/MS

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2013 年 24 卷 3 期

页码：

收录情况： SCI

摘要： Using the direct competitive chemiluminescent enzyme immunoassay (CLEIA), a rapid, highly selective and sensitive method for carbofuran determination was developed. Several physicochemical parameters such as chemiluminescent sensitiser, assay buffer, blocking substance and working concentration were optimised. Under the optimum conditions, the CLEIA method for carbofuran determination was generated successfully. The detection sensitivity of the IC50 value was 4.2 ng/mL at a practical working concentration ranging from 0.4 to 50 ng/mL, and the limit of detection (LOD) for carbofuran was 0.5 ng/mL. Recoveries of carbofuran spiked into vegetable, fruit and environmental samples were determined by CLEIA after sample matrix effect testing. Finally, the confirmation test between CLEIA and LC-MS/MS was finished. The results showed that CLEIA method had higher sensitivity than enzyme-linked immunosorbent assay method, and the LOD of a variety of samples reached the carbofuran's maximum residue limit value laid down by Codex Alimentarius Commission.

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