Brusatol isolated from Brucea javanica (L.) Merr. induces apoptotic death of insect cell lines

文献类型： 外文期刊

第一作者： Feng, Xuehuan

作者： Feng, Xuehuan;Ma, Dejun;Yang, Jingjing;Jiang, Hongyun;Zhang, Yanning;He, Weizhi

作者机构：

关键词： Brusatol;Insecticidal activities;Apoptosis;Spodoptera exigua;Brucea javanica

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2013 年 107 卷 1 期

页码：

收录情况： SCI

摘要： Brucea javanica (L.) Merr. is a medicine plant distributed widely throughout Asia where its bitter fruits have been used traditionally in medicine for treating various ailments and controlling some pests. In recent years, concerns over the potential impact of synthetic pesticides on human health and environment have now become more pressing to develop environmentally friendly pesticides. In this paper, brusatol, a quassinoid, was isolated from the fruit of B. javanica, and identified using X-ray crystallographic analysis. Results showed that brusatol has potent contact toxicity (LD50, 2.91 mu g/larva, 72 h) and anfieedant activity (AFC(50), 17.4 mg/L, 48 h) against the third-instar larvae of Spodoptera exigua. Brusatol demonstrated cytotoxic effects to the tested insect cell lines, IOZCAS-Spex-II and Sf21, in a time- and dose-dependent manner. After brusatol treatment, apoptotic cell death with the DNA fragmentation, activation of caspase-3 and release of cytochrome c was preliminarily observed in both IOZCAS-Spex-II and Sf21. These results indicated the existence of apoptotic death with the mitochondrial-dependent pathway induced by brusatol in Sf21 and IOZCAS-Spex-II cell lines. Our studies will provide important knowledge to understand mechanisms of action of brusatol and to develop brusatol and its derivatives as insecticides. (C) 2013 Elsevier Inc. All rights reserved.

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