Use of a pooled clone method to isolate a novel Bacillus thuringiensis Cry2A toxin with activity against Ostrinia furnacalis

文献类型： 外文期刊

第一作者： Shu, Changlong

作者： Shu, Changlong;Zhang, Jingtao;Chen, Guihua;Liang, Gemei;He, Kanglai;Huang, Dafang;Zhang, Jie;Song, Fuping;Shu, Changlong;Zhang, Jie;Zhang, Jingtao;Huang, Dafang;Crickmore, Neil

作者机构：

关键词： Bacillus thuringiensis;Cry2Ah1;Cry1Ac-resistance;Helicoverpa armigera

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2013 年 114 卷 1 期

页码：

收录情况： SCI

摘要： A pooled clone method was developed to screen for cry2A genes. This metagenomic method avoids the need to analyse isolated Bacillus thuringiensis strains by performing gene specific PCR on plasmid-enriched DNA prepared from a pooled soil sample: Using this approach the novel holotype gene cry2Ah1 was cloned and characterized. The toxin gene was over-expressed in Escherichia coli Rosetta (DE3) and the expressed toxin accumulated in both the soluble and insoluble fractions. The soluble Cry2Ah1 was found to have a weight loss activity against Ostrinia furnacalis, and a growth inhibitory activity to both Cry1Ac-susceptible and resistant Helicoverpa armigera populations. (C) 2013 Elsevier Inc. All rights reserved.

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