An insecticidal protein from Xenorhabdus ehlersii stimulates the innate immune response in Galleria mellonella

文献类型： 外文期刊

第一作者： Zeng, HongMei

作者： Zeng, HongMei;Yang, XiuFen;Liu, Zheng;Qiu, Dewen

作者机构：

关键词： Insecticidal protein;Galleria mellonella;Immune response;Proteomic analysis

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2013 年 29 卷 9 期

页码：

收录情况： SCI

摘要： The bacteria Xenorhabdus spp. are entomopathogenic symbionts that can produce several toxic proteins that interfere with the immune system of insects. Recently, we purified the insecticidal protein XeGroEL from Xenorhabdus ehlersii and discovered that injection of XeGroEL into larvae of Galleria mellonella triggers strong immune responses. In this study, we determined the level of induction of several immune-responsive proteins that were secreted into the hemolymph using comparative proteomic analyses of hemolymph proteins from XeGroEL-challenged larvae. Additionally, quantitative real-time reverse transcription-PCR analyses demonstrated increased transcriptional rates of immune-related genes at 5 h post-challenge with purified XeGroEL. Our results help to understand anti-microbial immune responses in G. mellonella, suggesting that the immune system recognizes exogenous proteins and pathogen-associated molecular patterns.

分类号：

  • 相关文献

[1]An insecticidal protein from Xenorhabdus budapestensis that results in prophenoloxidase activation in the wax moth, Galleria mellonella. Zeng, Hong-Mei,Yang, Xiu-Fen,Liu, Zheng,Guo, Li-Hua,Yuan, Jing-Jing,Qiu, De-Wen,Yang, Jun,Lin, Hua-Feng,Yang, Jun,Zeng, Hong-Mei,Yang, Xiu-Fen,Liu, Zheng,Guo, Li-Hua,Yuan, Jing-Jing,Qiu, De-Wen. 2012

[2]Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness. Liu, Dong,Liu, Zeng-Shan,Hu, Pan,Li, Yan-Song,Lu, Shi-Ying,Liu, Nan-Nan,Ma, Xiao-Long,Chi, Dan,Chang, Jiang,Shui, Yi-Ming,Li, Zhao-Hui,Ahmad, Waqas,Zhou, Yu,Ren, Hong-Lin,Fu, Bao-Quan,Cai, Ling.

[3]Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system. Khan, Sehroon,Xu, Jianchu,Khan, Sehroon,Xu, Jianchu,Khan, Sehroon,Guo Lihua,Qiu Dewen,Nadir, Sadia,Nadir, Sadia,Holmes, Keith A..

[4]Effect of chlorpyrifos on infectivity and survival of Steinernema feltiae. Chen, SL,Han, XY,Moens, M. 2003

[5]Variability of endotoxin expression in Bt transgenic cotton. Dong, H. Z.,Li, W. J.. 2007

[6]Effect of N-terminal and C-terminal Deletion of Cry1Ie of Bacillus thuringiensis on its Expression and Purification. Zhang, Chunlu,Guo, Shuyuan,Zhang, Jie,Song, Fuping. 2009

[7]Bacillus thuringiensis Vip3 mutant proteins: Insecticidal activity and trypsin sensitivity. Li, Chunyu,Xu, Ning,Huang, Xiaoping,Wang, Wei,Cheng, Jiaan,Wu, Kongming,Shen, Zhicheng. 2007

[8]A toxin protein from Xenorhabdus nematophila var. pekingensis and insecticidal activity against larvae of Helicoverpa armigera. Yang, Xiufen,Qiu, Dewen,Zhang, Yuliang,Zeng, Hongmei,Liu, Zheng,Yuan, Jingjing,Yang, Huaiwen. 2009

[9]Laboratory and Field Assessments of Prey-Mediated Effects of Transgenic Bt Rice on Ummeliata insecticeps (Araneida: Linyphiidae). Chen, Y.,Chen, X. X.,Hu, C.,Ye, G. Y.,Liu, Z. C.,Chen, M.,Peng, Y. F..

[10]Long photoperiod affects the maize transition from vegetative to reproductive stages: a proteomic comparison between photoperiod-sensitive inbred line and its recurrent parent. Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Song, Xiaoheng,Liu, Ping,Chen, Zan,Chen, Yanhui,Wu, Liuji,Zhang, Jun. 2018

[11]Quantitative Proteomic Analysis of Duck Ovarian Follicles Infected with Duck Tembusu Virus by Label-Free LC-MS. Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin,Han, Kaikai,Zhao, Dongmin,Liu, Yuzhuo,Liu, Qingtao,Huang, Xinmei,Yang, Jing,An, Fengjiao,Lin, Yin. 2016

[12]Comparative Proteomic Analysis of the Response of Maize (Zea mays L.) Leaves to Long Photoperiod Condition. Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Wu, Liuji,Tian, Lei,Wang, Shunxi,Liu, Ping,Tian, Zhiqiang,Zhang, Huimin,Chen, Yanhui,Zhang, Jun,Liu, Haiping. 2016

[13]Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses. Zhao, Dongming,Liang, Libin,Li, Yanbing,Liu, Liling,Guan, Yuntao,Jiang, Yongping,Chen, Hualan.

[14]Quantitative proteomic analysis of pre- and post-harvest peach fruit ripening based on iTRAQ technique. Wu, Xiaoqin,Mason, Aminah Myriah,Yu, Zhifang,Yu, Mingliang,Ma, Ruijuan.

[15]Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis. Chen, Ting,Xi, Qian-Yun,Sun, Jia-Jie,Ye, Rui-Song,Cheng, Xiao,Sun, Rui-Ping,Wang, Song-Bo,Shu, Gang,Wang, Li-Na,Zhu, Xiao-Tong,Jiang, Qing-Yan,Zhang, Yong-Liang,Sun, Rui-Ping. 2017

[16]Proteomic analysis reveals growth inhibition of soybean roots by manganese toxicity is associated with alteration of cell wall structure and lignification. Chen, Zhijian,Yan, Wei,Sun, Lili,Tian, Jiang,Liao, Hong,Chen, Zhijian,Yan, Wei,Sun, Lili,Liao, Hong.

[17]Evaluation of four protein extraction methods for proteomic analysis of mango peel. Liao, D. J.,Lu, X. P.,Chen, H. S.,Mo, Z. Y.,Lu, Y.. 2016

[18]Proteome profile of bovine ruminal epithelial tissue based on GeLC-MS/MS. Yang, Yongxin,Wang, Jiaqi,Yuan, Tingjie,Bu, Dengpan,Yang, Jinhui,Sun, Peng,Yang, Yongxin.

[19]iTRAQ Protein Profile Differential Analysis of Dormant and Germinated Grassbur Twin Seeds Reveals that Ribosomal Synthesis and Carbohydrate Metabolism Promote Germination Possibly Through the PI3K Pathway. Zhang, Guo-Liang,Zhu, Yue,Fu, Wei-Dong,Zhang, Rui-Hai,Zhang, Yan-Lei,Song, Zhen,Zhu, Yue,Wang, Peng,Xia, Gui-Xian,Wu, Jia-He.

[20]Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels. Li, Guangyu,Qiao, Qin,He, Yan,Zhang, Xuezhen,Yan, Wei,Chen, Jun,Cai, Fei. 2012

作者其他论文 更多>>

微信小程序