Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library
文献类型: 外文期刊
第一作者: Qin, Yong-Li
作者: Qin, Yong-Li;Sun, En-Cheng;Liu, Ni-Hong;Yang, Tao;Xu, Qing-Yuan;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Feng, Yu-Fei;Li, Jun-Ping;Wu, Dong-Lai;Qin, Yong-Li;Sun, En-Cheng;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Li, Jun-Ping
作者机构:
关键词: Bluetongue virus;NS2 protein;Monoclonal antibody;Epitope
期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )
ISSN: 0165-2427
年卷期: 2013 年 154 卷 3-4 期
页码:
收录情况: SCI
摘要: The NS2 protein of Bluetongue virus (BTV) is an important non-structural protein and plays important roles in viral replication and assembly. In this study, one monoclonal antibody (mAb), 4D4, was raised against BTV8 NS2. Phage display technology was used and identified the consensus binding motif SNYD recognized by mAb 4D4. To define the minimal region required for antibody binding, a panel of synthetic peptides encompassing SNYD derived from the BTV8 NS2 was then used to more specifically define the 4D4 epitope as (RSNYDV154)-R-149. Furthermore, amino acid sequence alignments of different BTV serotypes and other orbiviruses suggested that this epitope is highly conserved among the BTV serotypes. The mAb reagent generated in this study may be applied to the development of BTV diagnosis and surveillance programs and the epitope defined here can lead to important insights into how BTV might interact with the sheep's immune system. (C) 2013 Elsevier B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Integrative Analysis of Metabolome and Transcriptome of Carotenoid Biosynthesis Reveals the Mechanism of Fruit Color Change in Tomato (Solanum lycopersicum)
作者:Hu, Jiahui;Yu, Qinghui;Hu, Jiahui;Wang, Juan;Muhammad, Tayeb;Yang, Tao;Li, Ning;Yang, Haitao;Yu, Qinghui;Wang, Baike
关键词:tomato; fruit ripening; metabolome; transcriptome; carotenoids; lycopene; ethenyl
-
Comprehensive genomic characterization and expression analysis of calreticulin gene family in tomato
作者:Muhammad, Tayeb;Yang, Tao;Wang, Baike;Yang, Haitao;Wang, Juan;Yu, Qinghui;Tuerdiyusufu, Diliaremu
关键词:tomato; CRT gene family; endoplasmic reticulum; bioinformatics; abiotic stress; gene expression
-
Vegetation Restoration Increases Soil Carbon Storage in Land Disturbed by a Photovoltaic Power Station in Semi-Arid Regions of Northern China
作者:Zhao, Wenjing;Zhao, Jing;Liu, Meiying;Zhao, Wenjing;Zhao, Jing;Liu, Meiying;Zhao, Wenjing;Zhao, Jing;Liu, Meiying;Gao, Yong;Li, Wenlong;Duan, Haiwen
关键词:photovoltaic power stations; vegetation restoration; soil organic carbon; soil organic carbon storage; soil stoichiometry
-
Overexpression of transcription factor FaMYB63 enhances salt tolerance by directly binding to the SOS1 promoter in Arabidopsis thaliana
作者:Wang, Shuaishuai;Jiang, Rongyi;Feng, Jian;Zou, Haodong;Han, Xiaohuan;Xie, Xingbin;Zheng, Guanghui;Fang, Congbing;Zhao, Jing;Wang, Shuaishuai
关键词:Fragaria x ananassa; MYB; Salt stress; Regulator
-
The influence of traceability label trust on consumers' traceability pork purchasing behavior: Based on the moderating effect of food safety identification
作者:Liu, Zengjin;Yu, Meilian;Yu, Zhuo;Zhao, Jing;Han, Xibing;Geng, Ning;Li, Caixia
关键词:
-
Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster
作者:Bi, Xiangdong;Wei, Peng;Dai, Wei;Zhang, Dajuan;Wang, Qian;Duan, Lijin;Chen, Rui
关键词:
-
Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water
作者:Lei, Xingmei;Li, Peipei;Zhao, Jing;Xu, Lingyuan;Gao, Song;Zhao, Yun;She, Yongxin;Jin, Fen;Wang, Jing;Zheng, Lufei;Jin, Maojun;El-Aty, A. M. Abd;El-Aty, A. M. Abd;Li, Jia;Hammock, Bruce D.;Hammock, Bruce D.;Jin, Maojun
关键词:Ethirimol; Monoclonal antibody; Recombinant full-length antibody; Recognition mechanism; Ic-ELISA