Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library

文献类型: 外文期刊

第一作者: Qin, Yong-Li

作者: Qin, Yong-Li;Sun, En-Cheng;Liu, Ni-Hong;Yang, Tao;Xu, Qing-Yuan;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Feng, Yu-Fei;Li, Jun-Ping;Wu, Dong-Lai;Qin, Yong-Li;Sun, En-Cheng;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Li, Jun-Ping

作者机构:

关键词: Bluetongue virus;NS2 protein;Monoclonal antibody;Epitope

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

ISSN: 0165-2427

年卷期: 2013 年 154 卷 3-4 期

页码:

收录情况: SCI

摘要: The NS2 protein of Bluetongue virus (BTV) is an important non-structural protein and plays important roles in viral replication and assembly. In this study, one monoclonal antibody (mAb), 4D4, was raised against BTV8 NS2. Phage display technology was used and identified the consensus binding motif SNYD recognized by mAb 4D4. To define the minimal region required for antibody binding, a panel of synthetic peptides encompassing SNYD derived from the BTV8 NS2 was then used to more specifically define the 4D4 epitope as (RSNYDV154)-R-149. Furthermore, amino acid sequence alignments of different BTV serotypes and other orbiviruses suggested that this epitope is highly conserved among the BTV serotypes. The mAb reagent generated in this study may be applied to the development of BTV diagnosis and surveillance programs and the epitope defined here can lead to important insights into how BTV might interact with the sheep's immune system. (C) 2013 Elsevier B.V. All rights reserved.

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