Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library

文献类型： 外文期刊

第一作者： Qin, Yong-Li

作者： Qin, Yong-Li;Sun, En-Cheng;Liu, Ni-Hong;Yang, Tao;Xu, Qing-Yuan;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Feng, Yu-Fei;Li, Jun-Ping;Wu, Dong-Lai;Qin, Yong-Li;Sun, En-Cheng;Zhao, Jing;Wang, Wen-Shi;Wei, Peng;Li, Jun-Ping

作者机构：

关键词： Bluetongue virus;NS2 protein;Monoclonal antibody;Epitope

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

ISSN： 0165-2427

年卷期： 2013 年 154 卷 3-4 期

页码：

收录情况： SCI

摘要： The NS2 protein of Bluetongue virus (BTV) is an important non-structural protein and plays important roles in viral replication and assembly. In this study, one monoclonal antibody (mAb), 4D4, was raised against BTV8 NS2. Phage display technology was used and identified the consensus binding motif SNYD recognized by mAb 4D4. To define the minimal region required for antibody binding, a panel of synthetic peptides encompassing SNYD derived from the BTV8 NS2 was then used to more specifically define the 4D4 epitope as (RSNYDV154)-R-149. Furthermore, amino acid sequence alignments of different BTV serotypes and other orbiviruses suggested that this epitope is highly conserved among the BTV serotypes. The mAb reagent generated in this study may be applied to the development of BTV diagnosis and surveillance programs and the epitope defined here can lead to important insights into how BTV might interact with the sheep's immune system. (C) 2013 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Isolation of a Bluetongue virus group-specific monoclonal antibody and application to a diagnostic competitive ELISA. Xu, Q. Y.,Sun, E. C.,Yang, T.,Li, J. P.,Sun, L.,Sun, J.,Zhang, Q.,Wang, H. X.,Bu, Z. G.,Wu, D. L.,Wei, T.,Xi, N.,Liu, E. Z.,Feng, Y. F.,Geng, H. W..

[2]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[3]Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Yu, Shenye,Zhao, Hailing,Wang, Huanan,Wang, Xiumei,Xu, Lili,Si, Wei,Chen, Liping,Zhang, Wanjiang,Liu, Siguo,Shao, Guangxi. 2013

[4]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

[5]Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralising monoclonal antibody 8E8. Yang, Decheng,Zhang, Chunyuan,Zhao, Lei,Zhou, Guohui,Wang, Haiwei,Yu, Li. 2011

[6]Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Han, Zongxi,Zhao, Fei,Shao, Yuhao,Liu, Xiaoli,Kong, Xiangang,Song, Yang,Liu, Shengwang.

[7]Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis. Kang, Kai,Guo, Kangkang,Zhang, Yanming,Wu, Jiang,Li, Weiwei,Lin, Zhi,Tang, Qinhai.

[8]Analysis of murine B-cell epitopes on bluetongue virus 12 nonstructural protein 1. Wang HaiXiu,Sun EnCheng,Xu QingYuan,Yang Tao,Zhang Qin,Feng YuFei,Li JunPing,Lv Shuang,Sun Liang,Sun Jing,Wu DongLai.

[9]In silico prediction and in vitro identification of bluetongue virus 4 VP5 protein B-cell epitopes. Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P.,Wu, D. L.,Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P..

[10]DNA vaccine prime and recombinant FPV vaccine boost: an important candidate immunization strategy to control bluetongue virus type 1. Li, Junping,Yang, Tao,Xu, Qingyuan,Sun, Encheng,Feng, Yufei,Lv, Shuang,Zhang, Qin,Wang, Haixiu,Wu, Donglai.

[11]Identification of four novel group-specific bluetongue virus NS3 protein B-cell epitopes. Zhang Qin,Sun EnCheng,Xu QingYuan,Yang Tao,Wang HaiXiu,Feng YuFei,Li JunPing,Lv Shuang,Wu DongLai. 2015

[12]MicroRNA expression profiling of primary sheep testicular cells in response to bluetongue virus infection. Du, Junzheng,Gao, Shandian,Tian, Zhancheng,Xing, Shanshan,Huang, Dexuan,Zhang, Guorui,Zheng, Yadong,Liu, Guangyuan,Luo, Jianxun,Chang, Huiyun,Yin, Hong,Yin, Hong.

[13]Identification of two novel BTV16-specific B cell epitopes using monoclonal antibodies against the VP2 protein. Wang, Wen-Shi,Sun, En-Cheng,Xu, Qing-Yuan,Yang, Tao,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai.

[14]Analysis of the miRNA expression profile in an Aedes albopictus cell line in response to bluetongue virus infection. Xing, Shanshan,Du, Junzheng,Gao, Shandian,Tian, Zhancheng,Zheng, Yadong,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[15]Nanoparticle-based bio-barcode assay for the detection of bluetongue virus. Yin, Hui-qiong,Jia, Min-xian,Yang, Shu,Zhang, Li-ying,Zhang, Qi-mo,Zhang, Jin-gang,Shi, Li-jun,Li, Gang,Wang, Shen-qi. 2011

[16]Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Wang, Wen-Shi,Sun, En-cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai. 2013

[17]Emergence of a Novel Bluetongue Virus Serotype, China 2014. Sun, E. C.,Huang, L. P.,Xu, Q. Y.,Wang, H. X.,Bu, Z. G.,Wu, D. L.,Xue, X. M.,Lu, P.,Li, W. J.,Liu, W..

[18]Phylogenetic Characterization Genome Segment 2 of Bluetongue Virus Strains Belonging to Serotypes 5, 7 and 24 Isolated for the First Time in China During 2012 to 2014. Yang, H.,Xiao, L.,Wang, J.,Meng, J.,Liao, D.,Song, J.,Gao, L.,Xiong, H.,He, Y.,Li, H.,Lv, M.,Niu, B.,Chuang, X..

[19]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[20]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

作者其他论文 更多>>

微信小程序