Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hubner)

文献类型： 外文期刊

第一作者： Liu, Yang

作者： Liu, Yang;Wang, Guirong;Liu, Chengcheng;Dong, Shuanglin;Walker, William B.

作者机构：

关键词： Spodoptera exigua;Pheromone receptor gene;(Z,E)-9,12-tetradecadienyl acetate;(Z)-9-tetradecenol;Xenopus oocytes

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2013 年 43 卷 8 期

页码：

收录情况： SCI

摘要： In moths, males can detect a distinct blend of several pheromone components by specialized olfactory receptor neurons (ORNs) on the antennae. Four candidate pheromone receptors (PR) with seven transmembrane domains were identified by homology cloning from the antennae of Spodoptera exigua (Sexi). Phylogenetic analyses reveal that all four odorant receptors (OR) belong to pheromone receptor subtypes. Expression patterns revealed that PRs were male-specific in the antenna except for SexiOR11, which was female antenna-biased. Functional analyses of these PRs were conducted using heterologous expression in Xenopus oocytes. SexiOR13 and SexiOR16 were all broadly activated by multiple pheromone components. SexiOR13 responded robustly to the critical pheromone component, Z9, E12-14:OAc and the minor pheromone component, Z9-14:OAc at a concentration of 10(-4) M. Dose-response studies indicate that SexiOR13 was approximately 4 times more sensitive to Z9,E12-14:OAc (EC50 = 3.158 x 10(-6) M) compared to Z9-14:OAc (EC50 = 1.203 x 10(-5) M). While, SexiOR16 responded robustly to the secondary pheromone component Z9-14:OH with high sensitivity (EC50 = 9.690 x 10(-7) M). However, similar tests of the five pheromones with SexiOR6 and SexiOR11 failed to elicit any response. These results provide basic knowledge to further advance research on the molecular mechanisms of pheromone reception. (C) 2013 Elsevier Ltd. All rights reserved.

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