Differential activation of six galanin receptors by the spexin peptide in yellowtail kingfish ( Seriola lalandi)

文献类型： 外文期刊

第一作者： Wang, Bin

作者： Wang, Bin;Tian, Zhenfang;Yu, Zhihua;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Wang, Bin;Tian, Zhenfang;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Yu, Zhihua;Huang, Hai

作者机构：

关键词： Spexin; Galanin receptor; Signaling pathway; PKA; PKC; Ca2+

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：1.7； 五年影响因子：2.2 ）

ISSN： 0016-6480

年卷期： 2024 年 359 卷

页码：

收录情况： SCI

摘要： Spexin (SPX1) is a novel neuropeptide composed of 14 amino acids and well conserved across vertebrates, and it has been implicated in various physiological functions via galanin receptor 2 (GALR2) and GALR3. However, the detailed signaling pathways mediating its actions in target cells are still largely unknown. Accordingly, we addressed this issue in the present study using yellowtail kingfish as a model. SPX1 significantly increased CREluc activity in COS-7 cells expressing its cognate receptors GALR2a and GALR2b, and this stimulatory effect was attenuated by two inhibitors of the PKA pathway. Similarly, an evident induction of SRE-luc activity was observed when COS-7 cells transfected with GALR1b, GALR2a, GALR2b, GALR type 1, or GALR type 2 were challenged with SPX1, and two blockers of the PKC pathway suppressed this stimulatory action. Moreover, SPX1 markedly elevated NFAT-RE-luc activity in COS-7 cells expressing GALR1a, GALR2a, or GALR2b, and this promotion was inhibited by two antagonists of the Ca2+ route. Overall, our results have revealed that activation of six yellowtail kingfish galanin receptors by the SPX1 peptide may occur with different downstream signaling events, which could account for its pleotropic functions.

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