Comparative characterization of microRNA profiles of different genotypes of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Xu, M. J.

作者： Xu, M. J.;Zhou, D. H.;Huang, S. Y.;Zhao, F. R.;Song, H. Q.;Zhu, X. Q.;Xu, M. J.;Zhou, D. H.;Huang, S. Y.;Zhao, F. R.;Song, H. Q.;Zhu, X. Q.;Nisbet, A. J.;Lin, R. Q.;Zhu, X. Q.

作者机构：

关键词： Toxoplasma gondii;genotype;toxoplasmosis;microRNA (miRNA);expression profile

期刊名称：PARASITOLOGY （ 影响因子：3.234； 五年影响因子：3.118 ）

ISSN： 0031-1820

年卷期： 2013 年 140 卷 9 期

页码：

收录情况： SCI

摘要： The present study compared the miRNA expression profiles of five Toxoplasma gondii strains, namely RH (Type I, ToxoDB10), TgXD (Type I, ToxoDB10), PRU (Type II, ToxoDB1), QHO (Type II, ToxoDB1) and TgC7 (ToxoDB9), by Solexa deep sequencing, bioinformatics analysis and real-time quantitative PCR. A total of 7, 15, 10, 12 and 10 miRNAs were found from RH, TgXD, PRU, QHO and TgC7 strains, respectively. Thirteen miRNAs were shared by three genotypes, with only one miRNA shared by all of the 5 strains and others shared by 2 or more strains. A large number of targets ranging from 1 to 185 were identified for commonly shared miRNAs and strain-specific miRNAs with complete or nearly complete complementarity. Functional prediction showed that these targets were mostly focused on catalytic activity (191 targets) and binding activity (183 targets). Nonetheless, the majority of targets and most of the miRNAs are related to the virulence or invasion proteins of different strains of T. gondii, including ROP and MIC, as well as some other proteins, such as AMA1, GRA and RHO. The present study characterized comparatively the miRNA profiles of 3 different genotypes of T. gondii, identified genotype-shared miRNAs and strain-specific miRNAs.

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