Molecular characterization and functional analysis of two new-1,4-endoglucanase genes (Ha-eng-2, Ha-eng-3) from the cereal cyst nematode Heterodera avenae

文献类型： 外文期刊

第一作者： Peng, D.

作者： Peng, D.;Huang, W.;Peng, H.;Wang, G.;Long, H.

作者机构：

关键词： cell wall degradation;cellulose;Heterodera avenae;RNA

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

ISSN： 0032-0862

年卷期： 2013 年 62 卷 4 期

页码：

收录情况： SCI

摘要： Parasitism genes encoding secretory proteins expressed in the pharyngeal glands of plant-parasitic nematodes play a crucial role in nematode parasitism of plants. Two new -1,4-endoglucanase genes (Ha-eng-2 and Ha-eng-3) expressed in the pharyngeal glands of the sedentary cyst nematode, Heterodera avenae, were cloned. Both of the predicted proteins have a putative signal peptide for secretion and a catalytic domain. Neither peptide linkers nor cellulose binding domains were present. In situ hybridization showed that the transcripts of Ha-eng-2 and Ha-eng-3 accumulated specifically in the two subventral gland cells of H.avenae. RT-PCR analysis confirmed that their transcriptions were strong in the preparasitic and early parasitic second-stage juveniles, and were undetectable at the late parasitic stages of the nematode. Cellulase activities of the recombinant proteins HA-ENG-2 and HA-ENG-3 were confirmed in vitro. Knocking down Ha-eng-2 using RNA interference reduced nematode infectivity by 40%. The results indicate that these -1,4-endoglucanases can be secreted into plant tissues and play an important role in the wall degradation of plant cells during penetration and the migration of second-stage juveniles in host roots.

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