Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry

文献类型: 外文期刊

第一作者: Zhao, Liang

作者: Zhao, Liang;Meng, Kun;Bai, Yingguo;Shi, Pengjun;Huang, Huoqing;Luo, Huiying;Wang, Yaru;Yang, Peilong;Yao, Bin;Zhao, Liang;Song, Wei

作者机构:

关键词: Achaetomium;xylanase;heterologous expression;catalytic efficiency;brewing

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN: 0021-8561

年卷期: 2013 年 61 卷 28 期

页码:

收录情况: SCI

摘要: This study identified two family-11 xylanase genes (xynC81 and xynC83) in Achaetomium sp. Xz-8, a thermophilic strain from a desert area with substantial xylanase activity, and successfully expressed them in Pichia pastoris. Their deduced amino acid sequences showed the highest identity of <= 90% to known fungal xylanases and of <= 62% with each other. The purified recombinant xylanases showed optimal activities at pH 5.5 and 60-65 degrees C and exhibited stability over pH 5.0-10.0 and temperatures at 55 degrees C and below. XynC81 had high catalytic efficiency (6082 mL/s/mg), and XynC83 was favorable for xylooligosaccharide production. Under simulated mashing conditions, combination of XynC83 and a commercial beta-glucanase improved the filtration rate by 34.7696, which is much better than that of Novozymes Ultraflo (20.71%). XynC81 and XynC83 had a synergistic effect on viscosity reduction (7.08%), which is comparable with that of Ultraflo (8.47%). Thus, XynC81 and XynC83 represent good candidates for application in the brewing industry.

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