Extraction of Polysaccharides from Saccharomyces cerevisiae and its Immune Enhancement Activity
文献类型: 外文期刊
第一作者: Wang, Hui
作者: Wang, Hui;Dong, Pengcheng;Luo, Yongjiang;Cheng, Fusheng;Zhang, Xia
作者机构:
关键词: Polysaccharides;extraction;immunoassay;response surface methodology;Saccharomyces cerevisiae
期刊名称:INTERNATIONAL JOURNAL OF PHARMACOLOGY ( 影响因子:0.751; 五年影响因子:1.187 )
ISSN: 1811-7775
年卷期: 2013 年 9 卷 5 期
页码:
收录情况: SCI
摘要: Microbial polysaccharides are located both inside the fungal cell walls and on the fungal cell surfaces and possess marked immunological properties. In this study, the extraction conditions of Saccharomyces cerevisiae polysaccharides (SCPS) were optimized using response surface methodology and the immune enhancement properties of SCPS were evaluated. The results indicated that the optimal extraction conditions were high pressure homogenization time of 35 min, ultrasonic power of 510 W and ultrasonic time of 26 min, respectively. Under these conditions, the maximal observed value extraction yield of S. cerevisiae polysaccharides (SCPS) was (29.84 +/- 0.09)%, which was agreed with predicted value 29.82%. Thin layer chromatography (TLC) exhibited SCPS may be a glucan. Pharmacological experiments indicated that SCPS could increase alkaline phosphatase (AKP), lysozyme (LZM), immunoglobulin A (IgA), immunoglobulin G (IgG) and Epidermal Growth Factor (EGF) levels in serum, increase secreted immunoglobulin A (SIgA) expression in jejunum secretion and decrease prostaglandin E-2 (PGE(2)) expression in colon of immune-compromised rats at medium-dose. Saccharomyces cerevisiae polysaccharides has significant immune enhancement activity and could obviously protect intestine mucosa of immune-compromised rats.
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