Correlation between 146S Antigen Content in Foot-and-Mouth Disease Inactivated Vaccines and Immunogenicity Level and Vaccine Potency Alternative Test Methods

文献类型： 外文期刊

第一作者： Li, Yongxia

作者： Li, Yongxia;Zeng, Qiaoying;Li, Yongxia;Yang, Ruai;Yin, Fu;Zhang, Haisheng;Zhai, Guoyuan;Tian, Bo;Sun, Shiqi

作者机构：

关键词： foot-and-mouth disease inactivated vaccine; 146S content; antibody titer; qualification rate of antibody titer; PD50; IFN-gamma

期刊名称：VETERINARY SCIENCES （ 影响因子：2.4； ）

ISSN：

年卷期： 2024 年 11 卷 4 期

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收录情况： SCI

摘要： To investigate the association between 146S antigen contents in FMD inactivated vaccines and levels of antiviral immunity, this study vaccinated 30 kg pigs with three batches of FMD types O and A bivalent inactivated vaccines. Antibody titers and interferon-gamma (IFN-gamma) secretion levels were measured on days 7, 14, 21, and 28 after primary immunization and on days 14 and 28 following booster immunization to assess associations between 146S contents and both antibody titers and IFN-gamma secretion levels. Furthermore, 30 kg pigs were vaccinated with 46 batches of FMD type O inactivated vaccines and challenged on day 28, after which PD50 values were determined to evaluate the association between 146S content and PD50. The findings suggested that antibody titers and IFN-gamma secretion levels at specific time points after immunization were positively associated with 146S contents. Additionally, 146S content showed a positive correlation with PD50, with greater PD50 values recorded for 146S contents ranging from 4.72 to 16.55 mu g/dose. This investigation established a significant association between the 146S content in FMD inactivated vaccines and induced immune response against FMDV, thereby emphasizing its critical role in vaccine quality control. The determination of 146S content could serve as a new method for potency testing, offering an alternative to animal challenge tests.

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