Intracellular delivery of artificial transcription factors fused to the protein transduction domain of HIV-1 Tat

文献类型： 外文期刊

第一作者： Zhao, Xinghui

作者： Zhao, Xinghui;Dong, Yunzhu;Guo, Junwei;Liu, Jie;Huang, Peitang;Dong, Dayong;Fan, Hongyan;Guo, Qiang;Yang, Xiuxu;Xu, Junjie;Li, Jianmin;Fu, Ling;Chen, Wei;Zhao, Zhanzhong;Liu, Jie

作者机构：

关键词： Artificial transcription factor (ATF);Zinc finger;SV40 promoter;Kriippel-associated box (KRAB) domain;TAT peptide;Protein transduction domain

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN： 1046-5928

年卷期： 2013 年 90 卷 1 期

页码：

收录情况： SCI

摘要： Protein transduction domains (PTDs), such as the TAT peptide derived from HIV Tat protein, may transduce macromolecules into cells. In the present study, the TAT peptide-fused artificial transcription factors (ATFs) were generated by fusion of the N-terminal TAT peptide with SV40 promoter-targeted three-fingered C2H2 zinc finger proteins and the KRAB transcriptional repression domain. The fusion proteins were then expressed in an E.coli system and purified by Ni-NTA affinity chromatography. The purified fusion proteins were tested on mammalian cell lines CHO DG44 and L929. TAT-ATF-S, which contains the zinc fingers that bind to the SV40 promoter with high specificity, exhibited the desired transcriptional repression activity to the reported genes, indicating the successful cellular delivery and desired conformation of TAT-ATF-S. Our study has provided a new strategy for intracellular ATF delivery. (C) 2013 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition. Zhang, Zhe,Zhang, Feng,Cheng, Zhi-jun,Lin, Qi-bing,Wu, Fu-qing,Wang, Jiu-lin,Wang, Jie,Guo, Xiu-ping,Zhang, Xin,Lei, Cal-lin,Zhao, Zhi-chao,Zhu, Shan-shan,Wan, Jian-min,Liu, Ling-long,Zhang, Huan,Wan, Jian-min.

[2]Study on characteristics of in vitro culture and intracellular transduction of exogenous proteins in fibroblast cell line of Liaoning cashmere goat. Hu, P. F.,Guan, W. J.,Li, X. C.,Zhang, W. X.,Li, C. L.,Ma, Y. H.,Hu, P. F..

[3]In vitro inhibition of the replication of classical swine fever virus by capsid-targeted virus inactivation. Wang, Yu-Fei,Wang, Zhong-Hua,Li, Yan,Zhang, Xing-Juan,Sun, Yuan,Li, Miao,Qiu, Hua-Ji. 2010

作者其他论文 更多>>

微信小程序