Inactivation of the ABC transporter ATPase gene in Brucella abortus strain 2308 attenuated the virulence of the bacteria

文献类型： 外文期刊

第一作者： Zhang, Min

作者： Zhang, Min;Liu, Zongping;Zhang, Min;Han, Xiangan;Liu, Haiwen;Tian, Mingxing;Ding, Chan;Song, Jun;Sun, Xiaoqing;Yu, Shengqing

作者机构：

关键词： Brucella abortus;The ABC transporter ATPase gene;Gene inactivation;Virulence

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2013 年 164 卷 3-4 期

页码：

收录情况： SCI

摘要： Brucella abortus is a Gram-negative, facultative intracellular bacterial pathogen of human and other animals. Brucella lipopolysaccharide has been identified as an important virulence factor. In this study, the ABC transporter ATPase gene (BAB1_0542) of B. abortus strain S2308 was inactivated by deleting a 446-bp fragment from the gene, thereby generating the mutant strain, S2308 Delta AT. Real time PCR analysis confirmed the inactivation of this gene with no polar effect on the transcription of adjacent genes on the chromosome. The mutant was identified as a rough phenotype strain using heat agglutination test and crystal violet staining. The mutant strain had a different growth rate in Tryptic Soy Broth (TSB), compared to the wild type S2308 strain. Moreover, the mutant strain showed attenuated virulence in vitro and in vivo in RAW264.7 macrophages and Balb/c mice, respectively. Complementation of the mutant strain recovered the smooth phenotype of the bacteria and the complemented strain C2308 Delta ATP survived for more than four weeks in Balb/c mice, comparable to wild type strain S2308. Furthermore, immunization with the mutant strain protected mice from virulent strain challenge, which suggests the potential for the mutant strain S2308 Delta ATP as a future vaccine candidate. MHC 1, MHC II and co-stimulatory molecule expression levels in mice following infection of S2308 Delta ATP and S2308 were also investigated. (c) 2013 Elsevier B.V. All rights reserved.

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