AKT signalling and mitochondrial pathways are involved in mushroom polysaccharide-induced apoptosis and G(1) or S phase arrest in human hepatoma cells

文献类型: 外文期刊

第一作者: OuYang, Fengju

作者: OuYang, Fengju;Wang, Guibin;Zhang, Yuanyuan;Zhao, Min;Guo, Wei;Xiang, Wenhua

作者机构:

关键词: Medicinal mushrooms;Apoptosis;Cell cycle;Human hepatoma cells

期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )

ISSN: 0308-8146

年卷期: 2013 年 138 卷 4 期

页码:

收录情况: SCI

摘要: This study describes molecular mechanisms for inhibiting tumour cell proliferation using polysaccharides from medicinal mushrooms in human hepatoma cells. The results show that regarding cell cycle-related proteins, three types of polysaccharides significantly enhance the expression of p27(Kip) in HepG2 and Bel-7404 cells, while suppressing the activity of cyclin D1/CDK4 and/or cyclin E/CDK2. Considering apoptosis-related factors, the polysaccharides suppressed ART activity through the inhibition of ART phosphorylation at Thr(308) and/or Ser(473). The growth of HepG2 and Bel-7404 cells was suppressed by the up-regulation of a subunit of PI3K and phospho-PTEN, which are modulators of AKT activity. The polysaccharides also activated the mitochondria-mediated apoptosis pathway by stimulating the activation of BcI-2 family proteins to release cytochrome c and Smac and cleave caspase-9 and caspase-3 in HepG2 and Bel-7404 cells. These factors have a potent effect on cell cycle arrest in G(1) and/or S phase and induce apoptosis in HepG2 and Bel-7404 cells. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

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