Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

文献类型: 外文期刊

第一作者: Pei, Jie

作者: Pei, Jie;Lang, Xia;Guo, Xian;Bao, Pengjia;Liang, Chunnian;Chu, Min;Feng, Ruilin;Yan, Ping;Pei, Jie;Lang, Xia;Guo, Xian;Bao, Pengjia;Liang, Chunnian;Chu, Min;Yan, Ping

作者机构:

关键词: cattle;yak;growth hormone receptor;insulin-like growth factor-I;quantitative real-time PCR

期刊名称:ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING ( 影响因子:1.528; 五年影响因子:1.723 )

ISSN: 0003-9438

年卷期: 2013 年 56 卷

页码:

收录情况: SCI

摘要: The goals of this study were to compare the mRNA expression profiles of growth hormone receptor (GHR) and insulin-like growth factor I (IGF-I) in various tissues of cattle and the semi-wild yak (Datong yak) and to find out whether the mRNA levels of the two genes are correlated. The mRNA levels of GHR and IGF-I in heart, lung, liver, spleen, pancreas, kidney, muscle, mammary gland and ovary of cattle and yak were investigated by using quantitative real-time polymerase chain reaction (PCR). The experiments showed that the transcript levels of the two genes were significantly higher in liver (P<0.05) than in the other tissues for both species and that IGF-I levels varied more among tissues (P<0.01) than did GHR levels. The GHR tran script level in pancreas was higher in yak (P<0.05) than in cattle. There was no statistically significant difference in IGF-I tran script levels among all the tissues of both bovine groups. Growth hormone receptor and IGF-I transcript levels were positively correlated in mammary gland (P<0.01), lung (P<0.05) and muscle (P<0.05) in yak, negatively correlated in cattle heart (P<0.05) and not correlated in the other tissues. The results indicate that the two genes are regulated differently in various tissues under normal physiological conditions in these two bovine species.

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