Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China

文献类型： 外文期刊

第一作者： Wang, Feng-Xue

作者： Wang, Feng-Xue;Guo, Li;Yang, Yan-Ling;Song, Ni;Chen, Li-Zhi;Cheng, Shi-Peng;Wen, Yong-Jun

作者机构：

关键词： Porcine reproductive and respiratory syndrome virus;High pathogenicity;Isolation;Sequencing analysis;Nonstructural protein 2;China

期刊名称：INDIAN JOURNAL OF VIROLOGY （ 影响因子：0.364； 五年影响因子：0.287 ）

ISSN： 0970-2822

年卷期： 2013 年 24 卷 1 期

页码：

收录情况： SCI

摘要： The aim of the present study was to determine the causative agent of infected swines in the Jilin province of China and assess its genetic characteristics. Virus was isolated from tissues suspected of being infected by porcine reproductive and respiratory syndrome virus (PRRSV) and inoculated onto MARC-145 cells. Virus detection was carried out by RT-PCR, immunofluorescence, electron microscopy and sequencing. The results showed that the isolate was the North American genotype PRRSV, termed the JL-04/12 strain, with a 15,320 bp genome. The homology of the amino acid sequences in two nonstructural proteins and GP2 to GP5, between strains JL-04/12 and HUN4, ranged from 97.2 to 99.3 %. However, JL-04/12 GP6 and N protein were identical in HP-PRRSV JXA1 and HUN4. JL-04/12 was characterized by two discontinuous deletions in Nsp2. We speculate that the isolate is a variant of highly pathogenic porcine reproductive and respiratory syndrome derived from strains in 2006-2008. Altogether, these results indicate that highly pathogenic porcine reproductive and respiratory syndrome virus still exists in the Jilin province of China.

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