Isolation, characterization and expression analysis of the genes-GhAOS, GhAOC and GhOPR3: Encoding the key enzymes involved in jasmonic acid biosynthesis in Gladiolus hybridus

文献类型： 外文期刊

第一作者： Lian, Qing-long

作者： Lian, Qing-long;Yin, Yi-lei;Lian, Qing-long;Yin, Yi-lei;Lian, Qing-long;Xin, Hai-bo;Li, Xiao-xin;Zhong, Xiong-hui;Yi, Ming-fang;Xin, Hai-bo

作者机构：

关键词： Gladiolus hybridus;Allene oxide synthase;Allene oxide cyclase;12-Oxo-phytodienoic acid reductase 3;Methyl jasmonate;Corm formation

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2013 年 154 卷

页码：

收录情况： SCI

摘要： Jasmonic acid (JA) plays a regulatory role in plant development such as tuber and corm formation and their responses to environmental stresses. The action of JA in regulating plant growth and stress responses often requires the elevation of endogenous levels by de novo synthesis. Allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase 3 (OPR3) are the key enzymes involved in jasmonic acid biosynthesis in plants. Here, we isolated the AOS, AOC and OPR3 genes (GhAOS, GhAOC and GhOPR3) in full length from in vitro developing corms of Gladiolus hybridus. The subcellular localization analysis indicated that both GhAOS and GhAOC were chloroplast localization proteins whereas GhOPR3 was localized within the peroxisome. Real-time quantitative PCR showed that GhAOS,GhAOC and GhOPR3 genes were expressed constitutively in all organs with different levels having a relatively higher level in corms and cormels. Additionally, we observed that MJ treatments contributed to increasing the amount of three genes, mRNA level and endogenous MJ content thus promoting corm formation and enlargement. These results revealed that GhAOS, GhAOC and GhOPR3 were genes of significant importance and their expression contributed to JA biosynthesis thus playing an important role in corm formation and enlargement. (c) 2013 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Promotion of nicotine biosynthesis in transgenic tobacco by overexpressing allene oxide cyclase from Hyoscyamus niger. Tang, Kexuan,Jiang, Keji,Pi, Yan,Hou, Rong,Sun, Xiaofen,Tang, Kexuan,Jiang, Keji,Jiang, Lili.

[2]Soybean GmAOC3 Promotes Plant Resistance to the Common Cutworm by Increasing the Expression of Genes Involved in Resistance and Volatile Substance Emission in Transgenic Tobaccos. Wu, Qian,Wang, Hui,Wang, Yongli,Yu, Deyue,Wu, Qian,Wang, Dagang,Zhang, Lei,Huang, Zhiping,Wu, Juanjuan.

[3]Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice. Zheng, Zhigang,Yang, Xiaoming,Zhu, Longfei,Wei, Hantian,Lin, Xinchun,Fu, Yaping. 2017

[4]Identification and characterization of (E)-beta-caryophyllene synthase and alpha/beta-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton. Xiao, Yutao,Zhang, Wanna,Wu, Juan,Guo, Yuyuan,Zhang, Yongjun,Huang, Xinzheng,Wu, Junxiang,Koellner, Tobias G.. 2013

[5]Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer. Zhang, Yi Tong,Chen, Si Xue,Yang, Ze Zhong,Liu, Chun Guang,Zhao, Dan Dan,Ma, Yu Kun,Song, Fu Qiang,Yang, Feng Shan,Zhang, Yu Liang,Yin, Guo Hua,Zhang, Yi Tong,Chen, Si Xue,Yin, Guo Hua,Lee, Samantha,Bennett, Joan W.,Yang, Ze Zhong,Liu, Chun Guang,Zhao, Dan Dan,Ma, Yu Kun,Song, Fu Qiang,Yang, Feng Shan. 2015

[6]Herbivore species, infestation time, and herbivore density affect induced volatiles in tea plants. Cai, Xiao-Ming,Sun, Xiao-Ling,Dong, Wen-Xia,Wang, Guo-Chang,Chen, Zong-Mao,Cai, Xiao-Ming,Wang, Guo-Chang.

[7]Molecular cloning and characterization of the promoter for the multiple stress-inducible gene BjCHI1 from Brassica juncea. Wu, Xue-Feng,Wang, Chun-Lian,Xie, En-Bei,Gao, Ying,Fan, Ying-Lun,Zhao, Kai-Jun,Wu, Xue-Feng,Liu, Pi-Qing.

[8]Transcriptional response of the catharanthine biosynthesis pathway to methyl jasmonate/nitric oxide elicitation in Catharanthus roseus hairy root culture. Zhou, Mei-Liang,Shao, Ji-Rong,Zhou, Mei-Liang,Wu, Yan-Min,Tang, Yi-Xiong,Zhu, Xue-Mei.

[9]The effect of MeJA on ethylene biosynthesis and induced disease resistance to Botrytis cinerea in tomato. Yu, Mengmeng,Shen, Lin,Zhao, Danying,Zheng, Yang,Sheng, Jiping,Fan, Bei.

[10]A robust sampling approach for identification and quantification of methyl jasmonate in leaf tissue of oilseed rape for analysis of early signaling in sclerotinia sclerotiorum resistance. Wei, Fang,Cheng, Ji-hua,Liu, Sheng-yi,Huang, Jun-yan,Dong, Xu-yan,Li, Ping-ping,Kong, Fan-pi,Wu, Yu,Li, Yan-hua,Chen, Hong,Wang, Zhan,Feng, Yu-qi.

[11]Molecular cloning and characterization of three isoprenyl diphosphate synthase genes from alfalfa. Sun, Yan,Zhou, He,Zhang, Ze,Kang, Junmei,Zhang, Tiejun,Yang, Qingchuan.

[12]The effects of host defence elicitors on betacyanin accumulation in Amaranthus mangostanus seedlings. Cao, Shifeng,Harrison, Dion K.,Joyce, Daryl C.,Cao, Shifeng,Liu, Ting,Jiang, Yueming,He, Shenggen,Joyce, Daryl C..

[13]MeJA induces chilling tolerance in loquat fruit by regulating proline and gamma-aminobutyric acid contents. Cao, Shifeng,Cai, Yuting,Zheng, Yonghua,Cao, Shifeng,Yang, Zhenfeng. 2012

[14]Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis. Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong,Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong. 2017

[15]Methyl jasmonate induced defense responses increase resistance to Fusarium oxysporum f. sp cubense race 4 in banana. Sun, Dequan,Lu, Xinhua,Hu, Yulin,Li, Weiming,Hong, Keqian,Xie, Jianghui,Mo, Yiwei,Cahill, David M.. 2013

[16]VvMJE1 of the grapevine (Vitis vinifera) VvMES methylesterase family encodes for methyl jasmonate esterase and has a role in stress response. Zhao, Nan,Chen, Xinlu,Chen, Feng,Lin, Hong,Lan, Suque,Lan, Suque,Jia, Qidong,Chen, Feng,Guo, Hong.

[17]Effects of postharvest methyl jasmonate treatment on aromatic volatile biosynthesis by 'Nanguoli' fruit at different harvest maturity stages. Qin, G. H.,Wei, S. W.,Tao, S. T.,Zhang, H. P.,Huang, W. J.,Yao, G. F.,Zhang, S. L.,Qin, G. H.,Wei, S. W.,Sha, S. F..

[18]Molecular cloning and expression analysis of the mevalonate diphosphate decarboxylase gene from the latex of Hevea brasiliensis. Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong,Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong.

[19]Ectopic Expression of a Proteinase Inhibitor I4 (MtPiI4) Gene from Medicago truncatula Confers Plant Resistance to Pseudomonas syringae pv. Tomato DC3000. Sun, Di,Zhu, Can Can,Wang, Lei,Yang, Lu,Yang, Zhi Min,Chen, Jian,Hu, Liang Bin,Zhou, Zhao Sheng.

[20]Comparative proteomic analysis of latex from Hevea brasiliensis treated with Ethrel and methyl jasmonate using iTRAQ-coupled two-dimensional LC-MS/MS. Zeng, Rizhong.

作者其他论文 更多>>

微信小程序