Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174

文献类型： 外文期刊

第一作者： Yu, Shenye

作者： Yu, Shenye;Zhao, Hailing;Wang, Huanan;Wang, Xiumei;Xu, Lili;Si, Wei;Chen, Liping;Zhang, Wanjiang;Liu, Siguo;Shao, Guangxi

作者机构：

关键词： Monoclonal antibody;Phage phiX174;Protein E;Epitope

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2013 年 189 卷 2 期

页码：

收录情况： SCI

摘要： Bacterial ghosts offer a new avenue for the study of inactivated vaccines. However, for many years the mechanism of genetic inactivation was controversial. To obtain mouse monoclonal antibodies (mAbs) against protein E will allow the observation of its location and dynamic expression to expand understanding of the lysis mechanism. In this study, a His-tagged Delta E fusion protein expressed in bacteria was used as the immunogen, and mAbs targeting protein E were produced by the hybridoma technique and selected by enzyme-linked immunosorbent assay using GST-E and GST-AE as coating proteins. Purified mAbs from mouse ascites were screened against a phage-displayed random dodecapeptide library (Ph.D.-12). After three rounds of biopanning, 30 phage clones were randomly selected and sequenced, and their amino acids were deduced. One epitope showed a good match with protein E at 57-63aa (KPLN-R) and the synthetic decapeptide Epep (VRLKPLNCSR) strongly inhibited combination of E-A5 with E fusion proteins. Immunofluorescence microscopy indicated specific immunoreactivity of E-A5 with Escherichia coli-expressed native protein E. The novel mAbs may be of great potential value in analysis of the function and lysis pathway of protein E and other relative phages and in evaluation of E as potential marker of bacterial ghosts. (C) 2013 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[2]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

[3]Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralising monoclonal antibody 8E8. Yang, Decheng,Zhang, Chunyuan,Zhao, Lei,Zhou, Guohui,Wang, Haiwei,Yu, Li. 2011

[4]Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library. Qin, Yong-Li,Sun, En-Cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Feng, Yu-Fei,Li, Jun-Ping,Wu, Dong-Lai,Qin, Yong-Li,Sun, En-Cheng,Zhao, Jing,Wang, Wen-Shi,Wei, Peng,Li, Jun-Ping. 2013

[5]Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Han, Zongxi,Zhao, Fei,Shao, Yuhao,Liu, Xiaoli,Kong, Xiangang,Song, Yang,Liu, Shengwang.

[6]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[7]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

[8]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[9]Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity. Ding, Liangjun,Yuan, Qingyan,Xu, Pengfei,Yu, Yinhang,Li, Deshan,Hao, Zhichao. 2016

[10]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[11]SPIKE PROTEIN REGION (aa 636-789) OF PORCINE EPIDEMIC DIARRHEA VIRUS IS ESSENTIAL FOR INDUCTION OF NEUTRALIZING ANTIBODIES. Sun, D. B.,Feng, L.,Shi, H. Y.,Chen, J. F.,Liu, S. W.,Chen, H. Y.,Wang, Y. F..

[12]Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Guang-Zhi,Tian, Zhi-Jun,Liu, Jin-Xia,An, Tong-Qing,Peng, Jin-Mei,Cai, Xue-Hui,Wang, Mei,Wang, Yun-Feng,Xue, Qiang.

[13]Identifying Novel B Cell Epitopes within Toxoplasma gondii GRA6. Wang, Yanhua,Wang, Guangxiang,Cai, Jian Ping. 2016

[14]Phage display identifies an Eastern equine encephalitis virus glycoprotein E2-specific B cell epitope. Zhao, J.,Sun, E. C.,Liu, N. H.,Yang, T.,Xu, Q. Y.,Qin, Y. L.,Wu, D. L.,Zhao, J.,Sun, E. C.,Qin, Y. L.,Yang, Y. H.. 2012

[15]Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1. Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Zhang, Delin,Wang, Meng,Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Wang, Meng. 2013

[16]Critical Amino Acids Analysis of Epitopes in Pen a1. Mou Hui,Gao Mei-Xu,Wang Zhi-Dong,Zhao Jie,Li Shu-Jin,Zhao Xin,Li Shu-Rong,Pan Jia-Rong,Zhi Yu-Xiang. 2014

[17]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[18]Genotype-specific acquisition, evolution and adaptation of characteristic mutations in hepatitis E virus. Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua,Wang, Wenshi,Peppelenbosch, Maikel P.,Pan, Qiuwei,Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua. 2018

[19]In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua.

[20]Identification of a conserved B-cell epitope in the equine arteritis virus (EAV) N protein using the pepscan technique. Zhao, Shihua,Qi, Ting,Guo, Wei,Lu, Gang,Xiang, Wenhua.

作者其他论文 更多>>

微信小程序