Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis
文献类型: 外文期刊
第一作者: Yuan, Xianglian
作者: Yuan, Xianglian;Chen, Liping;Cao, Jun;Yu, Shenye;Liu, Siguo;Yuan, Xianglian;Deng, Xiaoxia;Pang, Hai;Wang Quankai
作者机构:
关键词: Mycobacterium tuberculosis;Rv0394c;Hyaluronidase;Chondrosulfatase
期刊名称:TUBERCULOSIS ( 影响因子:3.131; 五年影响因子:2.966 )
ISSN: 1472-9792
年卷期: 2013 年 93 卷 3 期
页码:
收录情况: SCI
摘要: It has been demonstrated that Mycobacterium tuberculosis can utilize hyaluronan (HA) as an alternative carbon resource; however, the gene responsible for HA utilization has not been identified. We overexpressed the soluble product of the Rv0394c gene from M. tuberculosis H37Rv in Escherichia coli and purified it using affinity chromatography and anion exchange chromatography. The hyaluronidase and chondrosulfatase activities of the purified recombinant protein Rv0394c (rRv0394c) were detected and quantitatively measured. Analysis of nucleotide and derived amino acid sequences of the Rv0394c gene revealed that homologs of this gene were conserved in pathogenic mycobacteria, but absent in nonpathogenic mycobacteria. In the current study, we provide novel identification and characterization of a gene encoding hyaluronidase and chondrosulfatase in M. tuberculosis. (C) 2013 Elsevier Ltd. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Improving UASS pesticide application: optimizing and validating drift and deposition simulations
作者:Tang, Qing;Zhang, Ruirui;Chen, Liping;Zhang, Pan;Li, Longlong;Xu, Gang;Yi, Tongchuan;Tang, Qing;Zhang, Ruirui;Chen, Liping;Zhang, Pan;Li, Longlong;Xu, Gang;Yi, Tongchuan;Hewitt, Andrew
关键词:lattice Boltzmann method (LBM); unmanned aerial spraying systems (UASS); Pest management; pesticide drift and deposition; optimization
-
Hyperspectral transmittance imaging detection of early decayed oranges caused by Penicillium digitatum using NFINDR-JMSAM algorithm with spectral feature separating
作者:Cai, Letian;Chen, Liping;Li, Xuetong;Zhang, Yizhi;Shi, Ruiyao;Li, Jiangbo;Cai, Letian
关键词:Citrus; Decay detection; Hyperspectral transmittance imaging; NFINDR-JMSAM; Spectral separation
-
Construction of a stable YOLOv8 classification model for apple bruising detection based on physicochemical property analysis and structured-illumination reflectance imaging
作者:Zhang, Junyi;Chen, Liping;Cai, Zhonglei;Shi, Ruiyao;Cai, Letian;Li, Jiangbo;Zhang, Junyi;Luo, Liwei;Yang, Xuhai;Li, Jiangbo
关键词:Apple; Bruising detection; Physicochemical property analysis; Structured-illumination reflectance imaging; Deep learning model
-
YOLO-detassel: Efficient object detection for Omitted Pre-Tassel in detasseling operation for maize seed production
作者:Yang, Jiaxuan;Zhang, Ruirui;Ding, Chenchen;Chen, Liping;Xie, Yuxin;Ou, Hong;Yang, Jiaxuan;Zhang, Ruirui;Ding, Chenchen;Chen, Liping;Xie, Yuxin;Ou, Hong;Yang, Jiaxuan;Chen, Liping
关键词:Detasseling; Object detection; UAV; Deep learning; Maize hybrid seed production
-
Boosting Cost-Efficiency in Robotics: A Distributed Computing Approach for Harvesting Robots
作者:Xie, Feng;Xie, Feng;Li, Tao;Feng, Qingchun;Li, Tao;Feng, Qingchun;Chen, Liping;Zhao, Chunjiang;Zhao, Hui
关键词:5G network; computation allocation; edge computing; harvesting robot; visual system
-
Combining dual-wavelength laser-induced fluorescence hyperspectral imaging with mutual information decomposition and redundancy elimination method to detect Aflatoxin B1 of individual maize kernels
作者:Fan, Yaoyao;Kang, Jian;Chen, Liping;Fan, Yaoyao;Yao, Xueying;Wang, Zheli;Long, Yuan;Chen, Liping;Huang, Wenqian;Tian, Xi;Tian, Xi
关键词:Dual-wavelength; Fluorescence hyperspectral imaging; Mutual information; Information decomposition; Maize kernels; Aflatoxin B1
-
Design and experiment of automatic docking system for liquid pesticide replenishment tube of field sprayer
作者:Gao, Yuanyuan;Feng, Kangyao;Wei, Xinhua;Han, Xin;Hu, Yongyue;Lu, Shengwei;Chen, Liping;Gao, Yuanyuan;Wei, Xinhua;Liu, Jingkai;Chen, Liping
关键词:Field sprayer; Autonomous operation; Liquid pesticide replenishment; Robotic arm; CAN bus; Visual servo; Positioning and docking