Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1

文献类型： 外文期刊

第一作者： Wang, Yanhua

作者： Wang, Yanhua;Wang, Guangxiang;Zhang, Delin;Yin, Hong;Zhang, Delin;Wang, Meng;Wang, Yanhua;Wang, Guangxiang;Zhang, Delin;Yin, Hong;Wang, Meng

作者机构：

关键词： Toxoplasma gondii;SAG1;Epitope;Pig antibodies

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2013 年 6 卷

页码：

收录情况： SCI

摘要： Background: The identification of protein epitopes is useful for diagnostic purposes and for the development of peptide vaccines. In this study, the epitopes of Toxoplasma gondii SAG1 were identified using synthetic peptide techniques with the aid of bioinformatics. Findings: Eleven peptides derived from T. gondii SAG1 were assessed by ELISA using pig sera from different time points after infection. Four (PS4, PS6, PS10 and PS11), out of the eleven peptides tested were recognized by all sera. Then, shorter peptides that were derived from PS4, PS6, PS10 and PS11 were predicted using bioinformatics and tested by experimentation. Four out of nine shorter peptides were identified successfully (amino acids 106-120, 166-180, 289-300 and 313-332). Conclusions: We have precisely located the epitopes of T. gondii SAG1 using pig sera collected at different time points after infection. The identified epitopes may be useful for the further study of epitope-based vaccines and diagnostic reagents.

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