Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal disease virus in chickens
文献类型: 外文期刊
第一作者: Li, Kai
作者: Li, Kai;Gao, Honglei;Gao, Li;Qi, Xiaole;Gao, Yulong;Qin, Liting;Wang, Yongqiang;Wang, Xiaomei
作者机构:
关键词: Infectious bursal disease virus;DNA vaccine;Chicken interleukin-18;Adjuvant
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2013 年 31 卷 14 期
页码:
收录情况: SCI
摘要: Interleukin-18 (IL-18) is an important cytokine with multiple functions in innate and acquired immunity. In this study, chicken IL-18 was evaluated for its adjuvant effects on the protective immunity of a DNA vaccine carrying the VP243 gene of IBDV. Groups of 14-day-old SPF chickens were given twice at 2-week intervals with 100 mu g of the plasmid DNA vaccine pCAGVP243, pCAGVP243-IL-18 and the blank vector pCAGGS, respectively, and challenged with vvIBDV (HLJ0504 strain) 2 weeks later. Chickens immunized with plasmid pCAGVP243-IL-18 carrying both VP243 and IL-18 genes induced significantly higher levels of antibodies, lymphocyte proliferation responses and of the cytokines IL-4 and IFN-gamma, than those injected with pCAGVP243 encoding the VP243 gene alone. Furthermore, pCAGVP243-IL-18 provided higher protection (93%) against vvIBDV challenge in chickens than pCAGVP243 (60%), as evidenced by the absence of clinical signs, mortality, and bursal atrophy. These results indicated that the cytokine IL-18 could enhance the immune responses and protection efficacy of DNA vaccine against IBDV infection in chickens, highlighting the potential value of chicken IL-18 as an adjuvant in the prevention of vvIBDV infection. (C) 2013 Published by Elsevier Ltd.
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