Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1

文献类型: 外文期刊

第一作者: Li, Wen Hui

作者: Li, Wen Hui;Jia, Wan Zhong;Qu, Zi Gang;Xie, Zhi Zhou;Luo, Jian Xun;Yin, Hong;Fu, Bao Quan;Sun, Xiao Lin;Blaga, Radu

作者机构:

关键词: Taenia multiceps;cytochrome c oxidase subunit 1 (cox1);genotype;phylogeny;China

期刊名称:KOREAN JOURNAL OF PARASITOLOGY ( 影响因子:1.341; 五年影响因子:1.669 )

ISSN: 0023-4001

年卷期: 2013 年 51 卷 2 期

页码:

收录情况: SCI

摘要: A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.

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