Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields
文献类型: 外文期刊
第一作者: Ding, Zai-Song
作者: Ding, Zai-Song;Huang, Su-Hua;Zhou, Bao-Yuan;Sun, Xue-Fang;Zhao, Ming
作者机构:
关键词: Phosphoenolpyruvate carboxylase;Transgenic rice;Photosynthesis;Yield;Drought
期刊名称:PLANT BIOTECHNOLOGY REPORTS ( 影响因子:2.01; 五年影响因子:1.907 )
ISSN: 1863-5466
年卷期: 2013 年 7 卷 2 期
页码:
收录情况: SCI
摘要: Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of CO2 in C-4 plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a C-4 SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed.
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