Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability

文献类型: 外文期刊

第一作者: Song, Zhang-yue

作者: Song, Zhang-yue;Tian, Jing-luan;Fu, Wei-zhe;Li, Lin;Lu, Ling-hong;Zhou, Lian;Shou, Hui-xia;Shan, Zhi-hui;Tang, Gui-xiang

作者机构:

关键词: Soybean;Agrobacterium-mediated transformation;Genotypes;Stable transgenic efficiency

期刊名称:JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B ( 影响因子:3.066; 五年影响因子:3.057 )

ISSN: 1673-1581

年卷期: 2013 年 14 卷 4 期

页码:

收录情况: SCI

摘要: The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and beta-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants.

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