Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Zeng, Hongmei

作者： Zeng, Hongmei;Qiu, Dewen;Liu, Xinqi

作者机构：

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS （ 影响因子：0.524； 五年影响因子：0.451 ）

ISSN： 1744-3091

年卷期： 2013 年 69 卷

页码：

收录情况： SCI

摘要： MoHrip2, a novel effector protein from the pathogenic fungus Magnaporthe oryzae, was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals and selenomethionine-labelled crystals were obtained using 2.2 M ammonium sulfate as a precipitant. A native data set was collected to 2.0 angstrom resolution at 100 K using an in-house X-ray source and a selenomethionine-labelled data set containing anomalous signal was collected to 1.8 angstrom resolution at 100 K using a synchrotron source. Based on the anomalous signal generated from the Se atom, the MoHrip2 structure was successfully solved using the single-wavelength anomalous dispersion (SAD) method.

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