The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia

文献类型： 外文期刊

第一作者： Liu, C.

作者： Liu, C.;Zhang, L. F.;Li, N.

作者机构：

关键词： Tibetan chicken;Hypoxia;Embryos;Globin;Hemoglobin

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY （ 影响因子：2.32； 五年影响因子：2.465 ）

ISSN： 1095-6433

年卷期： 2013 年 164 卷 4 期

页码：

收录情况： SCI

摘要： Tibetan chicken (Gallus gallus) is a specific chicken breed with strong ability to resist hypoxia, especially during embryonic stage. Though this breed has lived in Tibet plateau for thousands of years, the adaptive mechanism in response to hypoxia is still unknown. In order to obtain a better understanding of the mechanism of hypoxic adaptability in high altitude, we analyzed the mRNA expression pattern of globins in the present study. The fertilized eggs from Tibetan chicken and dwarf recessive white chicken breeds were incubated under normoxic (21% O-2) and hypoxic (13% O-2) conditions, equivalent to the altitude of 3600 m. We observed that Tibetan chicken embryos had higher hatchability (48%) in hypoxia than their lowland controls (7.8%). Northern blot showed that globin mRNA expression in Tibetan chicken embryos differed greatly from lowland controls under hypoxia. The expressions of four dominant globin mRNAs, named alpha(A), alpha(D), beta(A) and beta(H), were significantly induced under hypoxia. Tibetan chicken embryos had lower globin mRNA level in red cells than that of lowland controls at day 19 (P<0.05). Based on real-time PCR the same result was confirmed. Furthermore, we observed accumulation of globins induced by hypoxia in red cells by performing the separation of globin analysis, showing higher level of globins in red cells of Tibetan chicken embryos than that of lowland chicken embryos. Overall, our results provide new evidence that flexible regulation of globins at the level of transcription and translation may play a role in allowing the Tibetan chicken embryo to resist hypoxia. (C) 2012 Published by Elsevier Inc.

分类号：

  • 相关文献

[1]Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin. Xu, Sheng,Hu, Bing,He, Ziyi,Ma, Fei,Feng, Jianfei,Shen, Wenbiao,Yang, Jie. 2011

[2]Research on optic biosensor for rapid detection of hemoglobin. Baoshan He,Aiyu Zhou,Weiwei Yue,Liying Jiang,Chunxiu Liu,Xinxia Cai,Baoshan He,Aiyu Zhou,Weiwei Yue,Liying Jiang,Chunxiu Liu,Xinxia Cai. 2006

[3]Effects of iron from an amino acid complex on the iron status of neonatal and suckling piglets. Wei, KQ,Xu, ZR,Luo, XG,Zeng, LL,Chen, WR,Timothy, MF. 2005

[4]EFFECTS OF VITRIFICATION PROTOCOL ON THE LACTATE DEHYDROGENASE AND TOTAL ATPASE ACTIVITIES OF CHINESE MITTEN CRAB Eriocheir sinensis EMBRYOS. Huang, Xiaorong,Feng, Guangpeng,Zhao, Feng,Liu, Jianyi,Zhang, Tao,Wang, Yu,Zhuang, Ping. 2016

[5]Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa Mill.. Zhang, T,Cao, ZY,Wang, XY.

[6]Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro. Li Xiangchen,Yao Yaxin,Wang Yipeng,Li Heping,Guan Weijun,Ma Yuehui. 2012

[7]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[8]Dynamic analysis of Ca2+ level during bovine oocytes maturation and early embryonic development. Liang, Su Li,Zhao, Qian Jun,Li, Xiang Chen,Wang, Yi Peng,Su, Xiao Hua,Guan, Wei Jun,Ma, Yue Hui,Liang, Su Li,Jin, Ya Ping. 2011

[9]Developmental characteristics of pectoralis muscle in Pekin duck embryos. Gu, L. H.,Xu, T. S.,Sun, S. D.,Gu, L. H.,Xu, T. S.,Huang, W.,Xie, M.,Shi, W. B.,Hou, S. S.. 2013

[10]Research potential of multi-lineage chicken amniotic mesenchymal stem cells. Li, X.,Gao, Y.,Bian, Y.,Mu, R.,Guan, W.,Ma, Y.,Gao, Y.,Hua, J..

[11]Salinity stress on embryos and early larval stages of the pomfret Pampus punctatissimus. Zhang, Dong,Zhang, Dong,Shi, Zhaohong,Huang, Xuxiong,Fu, Rongbin,Wang, Haiping,Luo, Haizhong,Chen, Bo,Liu, Minghai.

[12]Screening for virus-resistant varieties of Chinese cherry in vitro. Shi, Y,Wang, Q,Zhou, P,Shi, C.

[13]Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos. Tian, Yongsheng,Jiang, Jing,Song, Lini,Chen, Zhangfan,Wang, Na,Chen, Songlin,Jiang, Jing,Song, Lini,Zhai, Jieming,Liu, Jiangchun.

[14]Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.). Gu, HH,Hagberg, P,Zhou, WJ.

[15]Immunization against inhibin improves in vivo and in vitro embryo production. Yan, Leyan,Li, Hui,Shi, Zhendan.

[16]Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice. Yu, Huifeng,Wark, Logan,Ji, Hua,Jaing, Yu,Han, Jing,Ortiz, Edlin,Zhang, Yunong,Medeiros, Denis M.,Lin, Dingbo,Ji, Hua,He, Hui,Willard, Lloyd,Medeiros, Denis M.. 2013

[17]Global gene expression responses to waterlogging in roots of sesame (Sesamum indicum L.). Wang, Linhai,Zhang, Yanxin,Qi, Xiaoqiong,Li, Donghua,Wei, Wenliang,Zhang, Xiurong. 2012

[18]Editorial: Eutrophication and hypoxia and their impacts on the ecosystem of the Changjiang Estuary and adjacent coastal environment. Zhang, Jing,Xiao, Tian,Huang, Daji,Liu, Su Mei,Fang, Jianguang. 2016

[19]Respiratory response of grass carp Ctenopharyngodon idellus to dissolved oxygen changes at three acclimation temperatures. Zhao, Zhigang,Xu, Qiyou,Dong, Shuanglin. 2018

[20]Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi. Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei.

作者其他论文 更多>>

微信小程序