Improved Resistance to Cucumber mosaic virus in Petunia Transformed with Non-Cytotoxic Pokeweed Antiviral Protein Gene

文献类型： 外文期刊

第一作者： Li, Yu

作者： Li, Yu;Chen, Dinghu;Wang, Xifeng;Feng, Hui;Chen, Dinghu

作者机构：

关键词： Petunia (Petunia hybrida);genetic transformation;pokeweed antiviral protein;China;Cucumber mosaic virus

期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）

ISSN： 0931-1785

年卷期： 2013 年 161 卷 4 期

页码：

收录情况： SCI

摘要： Petunia hybrida is an important ornamental plant that can be seriously affected by cucumber mosaic virus (CMV). Pokeweed antiviral protein (PAP), a ribosome-inactivating protein, has been recognized as a broad spectrum virus inhibitory agent. Mutant PAP efficiently inhibited viral gene expression at both the translational and transcriptional levels without causing host cell toxicity. We have transferred the non-cytotoxic pokeweed antiviral protein (mutant PAP) gene into petunia cells with Agrobacterium tumefaciens. Forty-two putative transgenic regenerated lines were obtained from the selected explants. Fifty-six plants immune to CMV infection were recovered from nine transgenic lines. Another 51 plants tolerant to CMV were obtained from 10 transgenic regenerated lines. Successful integration of the mutant PAP gene into the genome of transgenic petunia was confirmed by PCR and Southern blot analysis. Expression of the PAP gene was further confirmed by RT-PCR and Western blot analysis. These results were consistent with the assay of resistance to CMV.

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