Genotyping Cryptosporidium andersoni in Cattle in Shaanxi Province, Northwestern China

文献类型： 外文期刊

第一作者： Zhao, Guang-Hui

作者： Zhao, Guang-Hui;Ren, Wan-Xin;Zhu, Xing-Quan;Zhao, Guang-Hui;Ren, Wan-Xin;Bian, Qing-Qing;Hu, Bing;Cong, Mei-Mei;Lin, Qing;Qi, Mao-Zhen;Gao, Man;Wang, Rong-Jun;Qi, Meng;Zhang, Long-Xian

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 4 期

页码：

收录情况： SCI

摘要： The present study examined the prevalence and genotypes of Cryptosporidium andersoni in cattle in Shaanxi province, China. A total of 2071 fecal samples (847 from Qinchuan cattle and 1224 from dairy cattle) were examined for the presence of Cryptosporidium oocysts, and 70 samples (3.4%) were C. andersoni-positive and those positive samples were identified by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) and the Cryptosporidium oocyst wall protein (COWP) genes. C. andersoni was the only species found in the examined cattle in this province. Fifty-seven C. andersoni isolates were characterized into 5 MLST subtypes using multilocus sequence typing analysis, including a new subtype in the native beef breed Qinchuan cattle. All of these C. andersoni isolates presented a clonal genetic structure. These findings provide new insights into the genetic structure of C. andersoni isolates in Shaanxi province and basic data of Cryptosporidium prevalence status, which in turn have implications for controlling cryptosporidiosis in this province.

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