Determination of beta(2)-Agonists in Porcine Urine by Molecularly Imprinted Solid Phase Extraction Followed Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Detection
文献类型: 外文期刊
第一作者: Wang, Peilong
作者: Wang, Peilong;Li, Yang;Wang, Ruiguo;Zhang, Wei;Cheng, Jie;Wang, Jing;Su, Xiaoou;Ye, Zhihua;Wang, Peilong;Li, Yang;Wang, Ruiguo;Zhang, Wei;Cheng, Jie;Wang, Jing;Su, Xiaoou;Ye, Zhihua
作者机构:
关键词: 2-agonists;MIP;Porcine urine;UPLC-MS;MS
期刊名称:ANALYTICAL LETTERS ( 影响因子:2.329; 五年影响因子:1.738 )
ISSN: 0003-2719
年卷期: 2013 年 46 卷 5 期
页码:
收录情况: SCI
摘要: A novel, sensitive, and robust method has been developed to detect 9 2-agonists in porcine urine to monitor illegal use of 2-agonists in swine rearing. The method based on the molecular imprinted polymer (MIP) rapid extraction followed ultra-performance liquid chromatography coupled tandem mass spectrometry (UPLC-MS/MS) detection. The cleaning efficiency of MIP cartridges was demonstrated by comparing with common ion exchange solid phase extraction. The presented method was validated in accordance with the European Commission Decision 2002/657/EC. The linearity, decision limit (CC), detection capability (CC), recovery, precision, robustness, and stability were studied in detail. CC and CC values were from 0.006ng/mL to 0.03ng/mL and from 0.02ng/mL to 0.08ng/mL, respectively. The mean recoveries and repeatability varied from 68.8% to 94.2% and from 2.8% to 10.1%. The proposed method was applied to test 170 porcine urine samples from the Shaanxi province in China and two urine samples were confirmed as clenbuterol positive and the concentrations of clenbuterol in positive urine samples were about 0.08ng/mL and 0.1ng/mL, respectively. The developed method was demonstrated to be more sensitive and robust for the determination of 9 2-agonists in porcine urine. The method was proven to be simple and easy in operation with high selectivity and good reproducibility.
分类号:
- 相关文献
作者其他论文 更多>>
-
Comprehensive metabolic profiling of dioxin-like compounds exposure in laying hens: Implications for toxicity assessment
作者:Hu, Xiaoxu;Xu, Tong;Chen, Yangsheng;Zheng, Liping;Wang, Chu;Zhang, Qinghua;Xie, Heidi Qunhui;Xu, Li;Zhao, Bin;Hu, Xiaoxu;Xu, Tong;Chen, Yangsheng;Zheng, Liping;Wang, Chu;Zhang, Qinghua;Xie, Heidi Qunhui;Xu, Li;Zhao, Bin;Zhang, Qian;Tang, Lijuan;Wang, Pu;Dong, Shujun;Wang, Ruiguo;Zhang, Su
关键词:Polychlorinated dibenzo-p-dioxins; and furans; Dioxin-like polychlorinated; biphenyls; Laying hens; Metabolomic
-
Heavy rainfall stimulates more N2O emissions from wheat fields during basal and overwintering fertilization phases
作者:Wang, Jing;Liu, Qiang;Liu, Qiang;Zhao, Suya;He, Shiying;Yang, Bei;Wang, Ning;Hou, Pengfu;Xue, Lihong;Yang, Linzhang;Zhao, Suya;He, Shiying;Wang, Ning;Hou, Pengfu;Xue, Lihong
关键词:Rainfall; Nitrous oxide; Nitrogen transformation; Functional microorganisms
-
Soil bacterial diversity and community structure of Suaeda glauca vegetation in the Hetao Irrigation District, Inner Mongolia, China
作者:Dong, Ruixiao;Wang, Xinbo;Li, Yuyi;Zhang, Hongyuan;Li, Xiaobin;Song, Jiashen;Chang, Fangdi;Feng, Wenhao;Pang, Huancheng;Wang, Jing
关键词:Suaeda glauca; saline-alkali soil; rhizosphere; bacteria; community structure
-
Membralin is required for maize development and defines a branch of the endoplasmic reticulum-associated degradation pathway in plants
作者:Liu, Baiyu;Zhang, Ke;Qi, Shoumei;Jin, Zhe;Chen, Donghua;Zhang, Wei;Zhang, Kewei;Li, Kunpeng;Xu, Changzheng;He, Qiuxia;Cheng, Wen;Ding, Zhaohua;Zhao, Xiangyu
关键词:endoplasmic reticulum-associated degradation; maize; membralin; plant development; unfold protein response
-
Biocontrol performance and mass production potential of the larval endoparasitoid Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
作者:Zhang, Rui;Zhang, Wei;Zhao, Qi;Keyhani, Nemat O.;Lei, Xian-Fu;Liu, Chang-Hua;Al Dhafer, Hathal M.;Mohamed, Amr
关键词:Spodoptera frugiperda; Field investigation; Campoletis chlorideae; Biological control; Mass production; Two-sex life table
-
Identification of candidate gene associated with maize northern leaf blight resistance in a multi-parent population
作者:Bi, Yaqi;Jiang, Fuyan;Yin, Xingfu;Shaw, Ranjan K.;Guo, Ruijia;Wang, Jing;Fan, Xingming
关键词:Zea mays; Northern leaf blight; Multi-parent population; Genome-wide association analysis; QTL mapping; CATETO germplasm
-
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者:Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词: