Determination of beta(2)-Agonists in Porcine Urine by Molecularly Imprinted Solid Phase Extraction Followed Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Detection
文献类型: 外文期刊
第一作者: Wang, Peilong
作者: Wang, Peilong;Li, Yang;Wang, Ruiguo;Zhang, Wei;Cheng, Jie;Wang, Jing;Su, Xiaoou;Ye, Zhihua;Wang, Peilong;Li, Yang;Wang, Ruiguo;Zhang, Wei;Cheng, Jie;Wang, Jing;Su, Xiaoou;Ye, Zhihua
作者机构:
关键词: 2-agonists;MIP;Porcine urine;UPLC-MS;MS
期刊名称:ANALYTICAL LETTERS ( 影响因子:2.329; 五年影响因子:1.738 )
ISSN: 0003-2719
年卷期: 2013 年 46 卷 5 期
页码:
收录情况: SCI
摘要: A novel, sensitive, and robust method has been developed to detect 9 2-agonists in porcine urine to monitor illegal use of 2-agonists in swine rearing. The method based on the molecular imprinted polymer (MIP) rapid extraction followed ultra-performance liquid chromatography coupled tandem mass spectrometry (UPLC-MS/MS) detection. The cleaning efficiency of MIP cartridges was demonstrated by comparing with common ion exchange solid phase extraction. The presented method was validated in accordance with the European Commission Decision 2002/657/EC. The linearity, decision limit (CC), detection capability (CC), recovery, precision, robustness, and stability were studied in detail. CC and CC values were from 0.006ng/mL to 0.03ng/mL and from 0.02ng/mL to 0.08ng/mL, respectively. The mean recoveries and repeatability varied from 68.8% to 94.2% and from 2.8% to 10.1%. The proposed method was applied to test 170 porcine urine samples from the Shaanxi province in China and two urine samples were confirmed as clenbuterol positive and the concentrations of clenbuterol in positive urine samples were about 0.08ng/mL and 0.1ng/mL, respectively. The developed method was demonstrated to be more sensitive and robust for the determination of 9 2-agonists in porcine urine. The method was proven to be simple and easy in operation with high selectivity and good reproducibility.
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