Optimization of hydrolysis conditions for the production of glucomanno-oligosaccharides from konjac using beta-mannanase by response surface methodology

文献类型： 外文期刊

第一作者： Chen, Junfan

作者： Chen, Junfan;Liu, Desheng;Cheng, Yongqiang;Chen, Junfan;Liu, Desheng;Cheng, Yongqiang;Shi, Bo;Zhang, Wenjing;Wang, Hai

作者机构：

关键词： Glucomanno-oligosaccharides;Response surface methodology;Konjac;Enzymatic hydrolysis

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN： 0144-8617

年卷期： 2013 年 93 卷 1 期

页码：

收录情况： SCI

摘要： Glucomanno-oligosaccharides (GMO), usually produced from hydrolysis of konjac tubers with a high content of glucomannan, have a positive effect on Bifidobacterium as well as a variety of other physiological activities. Response surface methodology (RSM) was employed to optimize the hydrolysis time, hydrolysis temperature, pH and enzyme to substrate ratio (E/S) to obtain a high GMO yield from konjac tubers. From the signal-factor experiments, it was concluded that the change in the direct reducing sugar (DRS) is consistent with total reducing sugar (TRS) but contrary to the degree of polymerization (DP). DRS was used as an indicator of the content of GMO in the RSM study. The optimum RSM operating conditions were: reaction time of 3.4h, reaction temperature of 41.0 degrees C, pH of 7.1 and E/S of 0.49. The results suggested that the enzymatic hydrolysis was enhanced by temperature, pH and incubation time. Model validation showed good agreement between experimental results and the predicted responses. (C) 2012 Elsevier Ltd. All rights reserved.

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