Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs

文献类型: 外文期刊

第一作者: Cao, Yimei

作者: Cao, Yimei;Lu, Zengjun;Li, Yanli;Sun, Pu;Li, Dong;Li, Pinghua;Bai, Xingwen;Fu, Yuanfang;Bao, Huifang;Zhou, Chunxue;Xie, Baoxia;Chen, Yingli;Liu, Zaixin

作者机构:

关键词: FMDV;Multi-epitope protein vaccine;Poly(I:C);Pig

期刊名称:ANTIVIRAL RESEARCH ( 影响因子:5.97; 五年影响因子:5.801 )

ISSN: 0166-3542

年卷期: 2013 年 97 卷 2 期

页码:

收录情况: SCI

摘要: We designed a series of epitope proteins containing the G-H loops of three topotypes of foot-and-mouth disease virus (FMDV) serotype O and promiscuous artificial Th sites and selected one epitope protein (designated as B4) with optimal immunogenicity and cross-reactivity. Three out of five pigs immunized intramuscularly with this B4 were protected against virulent FMDV challenge after a single inoculation, while all pigs co-immunized with B4 and polyinosinic-cytidylic acid [poly(I:C)] conferred complete protection following FMDV challenge. Additionally, we demonstrated that all pigs co-immunized with B4 and poly(I:C) elicited FMDV-specific neutralizing antibodies, total IgG antibodies, type I interferon (IFN-alpha/beta) and cytokines IFN-gamma. In contrast, some pigs immunized with B4 alone produced parameters mentioned above, while some not, suggesting that poly(I:C) reduced animal-to-animal variations in both cellular and humoral responses often observed in association with epitope-based vaccines and up-regulated T-cell immunity often poorly observed in protein-based vaccines. We propose that poly(I:C) is an effective adjuvant for this epitope-based vaccine of FMDV. This combination could yield an effective and safe candidate vaccine for the control and eradication of FMD in pigs. (c) 2012 Elsevier B.V. All rights reserved.

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