Molecular and biochemical characterization of a new alkaline active multidomain xylanase from alkaline wastewater sludge

文献类型： 外文期刊

第一作者： Zhao, Yanyu

作者： Zhao, Yanyu;Meng, Kun;Luo, Huiying;Huang, Huoqing;Yuan, Tiezheng;Yao, Bin;Zhao, Yanyu;Yang, Peilong

作者机构：

关键词： Alkaline wastewater sludge;Biobleaching;Direct cloning;Glycoside hydrolase (GH) family 10

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2013 年 29 卷 2 期

页码：

收录情况： SCI

摘要： A xylanase gene, xyn-b39, coding for a multidomain glycoside hydrolase (GH) family 10 protein was cloned from the genomic DNA of the alkaline wastewater sludge of a paper mill. Its deduced amino acid sequence of 1,481 residues included two carbohydrate-binding modules (CBM) of family CBM_4_9, one catalytic domain of GH 10, one family 9 CBM and three S-layer homology (SLH) domains. xyn-b39 was expressed heterologously in Escherichia coli, and the recombinant enzyme was purified and characterized. Xyn-b39 exhibited maximum activity at pH 7.0 and 60 A degrees C, and remained highly active under alkaline conditions (more than 80 % activity at pH 9.0 and 40 % activity at pH 10.0). The enzyme was thermostable at 55 A degrees C, retaining more than 90 % of the initial activity after 2 h pre-incubation. Xyn-b39 had wide substrate specificity and hydrolyzed soluble substrates (birchwood xylan, beechwood xylan, oat spelt xylan, wheat arabinoxylan) and insoluble substrates (oat spelt xylan and wheat arabinoxylan). Hydrolysis product analysis indicated that Xyn-b39 was an endo-type xylanase. The K (m) and V (max) values of Xyn-b39 for birchwood xylan were 1.01 mg/mL and 73.53 U/min/mg, respectively. At the charge of 10 U/g reed pulp for 1 h, Xyn-b39 significantly reduced the Kappa number (P < 0.05) with low consumption of chlorine dioxide alone.

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[1]A xylanase gene directly cloned from the genomic DNA of alkaline wastewater sludge showing application potential in the paper industry. Zhao, Yanyu,Luo, Huiying,Meng, Kun,Shi, Pengjun,Wang, Guozeng,Yuan, Tiezheng,Yao, Bin,Zhao, Yanyu,Yang, Peilong.

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