Do-it-yourself Molecular Biology for Plant Breeding: Low-cost Tools for Developing Countries

文献类型： 外文期刊

第一作者： Huynh, O. A.

作者： Huynh, O. A.;Hofinger, B. J.;Jankowicz-Cieslak, J.;Forster, B. P.;Till, B. J.;Beshir, M. M.;Guo, H.

作者机构：

关键词： DNA extraction;mutation discovery;TILLING

期刊名称：PROCEEDINGS OF THE GENETIC TRANSFORMATION TECHNOLOGIES (PLANTS AND ANIMALS): PLANT GENETICS AND BREEDING TECHNOLOGIES: PLANT DISEASES AND RESISTANCE MECHANISMS

ISSN：

年卷期： 2013 年

页码：

收录情况： SCI

摘要： Technological advances in the field of genomics and phenomics in the past decade are cause for great optimism that the global challenge of sustainable food security can be met through crop improvements. Yet the task of transferring knowledge and methodologies from developed to developing countries remains difficult. Our group, the Plant Breeding and Genetics Laboratory of the FAO/IAEA Joint Programme, aims to assist developing countries in their efforts to improve crops through the use of induced mutations and associated biotechnologies. Using current high-throughput technologies from TILLING to Next Generation Sequencing, we are attempting to understand better and optimise mutagenesis in diverse crop genomes. At the same time, we are reverse-engineering and adapting assays to be better suited for developing countries that have varying levels of infrastructure and human capacity. How can this be accomplished? Basic issues need to be considered when developing methodologies such as costs, lack of consistent power, limited availability of equipment and reagents, and issues with hazardous waste disposal. Here we describe low cost and low toxicity methods for the collection of plant leaf material and subsequent extraction of DNA. Drying leaf material so that it can be stored at room temperature precludes the need for continuous power and high energy usage -80 degrees C freezers. Low cost and low toxicity DNA extraction can be performed for less than 20 US cents per sample. Combined with low cost methods for SNP and small indel discovery using self-extracted nucleases, low to medium throughput genotyping and TILLING can be implemented in many laboratories. Future challenges include reducing the cost and toxicity of agarose gel electrophoresis and subsequent imaging.

分类号：

  • 相关文献

[1]Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat. Zhai, Shengnan,Sun, Youwei,Li, Jihu,He, Zhonghu,Xia, Xianchun,Li, Genying,Song, Jianmin,Song, Guoqi,Li, Yulian,Ling, Hongqing,He, Zhonghu. 2016

[2]Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING. Li, Wenjie,Zhao, Baocun,Li, Wenjie,Guo, Huijun,Xie, Yongdun,Zhao, Linshu,Gu, Jiayu,Zhao, Shirong,Liu, Luxiang,Wang, Yongbin,Wang, Guangjin.

[3]Novel mutant alleles of the starch synthesis gene TaSSIVb-D result in the reduction of starch granule number per chloroplast in wheat. Guo, Huijun,Liu, Yunchuan,Li, Xiao,Yan, Zhihui,Xie, Yongdun,Xiong, Hongchun,Zhao, Linshu,Gu, Jiayu,Zhao, Shirong,Liu, Luxiang. 2017

[4]DNA extraction from crayfish exoskeleton. Li, Yanhe,Wang, Weimin,Liu, Xiaolian,Luo, Wei,Zhang, Jie,Gul, Yasmeen,Li, Yanhe. 2011

[5]A simplified genomic DNA extraction protocol for pre-germination genotyping in rice. Duan, Y. B.,Zhao, F. L.,Chen, H. D.,Sheng, W.,Teng, J. T.,Zhang, A. M.,Xue, J. P.,Li, H.,Ni, D. H.,Wei, P. C.. 2015

[6]Comparative analysis of different protocols for extraction of DNA from fish scales of Cyprinus carpio. Li, Yanhe,Gul, Yasmeen,Cui, Lei,Cao, Xiaojuan,Wang, Weimin,Li, Yanhe,Gul, Yasmeen. 2015

[7]Studies of the genetic diversity of seven sweetsop (Annona squamosa L.) cultivars by amplified fragment length polymorphism analysis. Zhao Zhichang,Hu Guibing,Ouyang Ruo,Liu Yunchun,Zhao Zhichang,Chen Yeyuan,Luo Shirong. 2011

[8]Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue. Liu, L.,Wang, C. L.,Peng, W. Y.,Yang, J.,Lan, M. Q.,Zhang, B.,Zhu, Y. Y.,Li, C. Y.,Li, J. B.. 2015

[9]Development and Evaluation of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Tylenchulus semipenetrans Using DNA Extracted from Soil. Zhang, De-Yong,Liu, Yong,Song, Zhi-Qiang,Cheng, Ju-E,Cheng, Fei-Xue,Zhang, De-Yong,Liu, Yong. 2017

[10]Comparison of Three DNA Extraction Methods for Feed Products and Four Amplification Methods for the 5 '-Junction Fragment of Roundup Ready Soybean. Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua. 2012

[11]Isolation and PCR detection of foreign DNA sequences in bee honey raised on genetically modified Bt (Cry1Ac) cotton. Jin, W.,Wu, H.,Wang, F.,You, C.,Peng, Y.,Jia, S.. 2007

作者其他论文 更多>>

微信小程序