A Comparison of in vitro Development of Buffalo (Bubalus bubalis) Embryos Produced either by Somatic Cell Nuclear Transfer or in vitro Fertilization using Oocytes Obtained by Different Methods

文献类型: 外文期刊

第一作者: Yang, Chunyan

作者: Yang, Chunyan;Pang, Chunying;Zheng, Haiying;Huang, Fenxiang;Shang, Jianghua;Wang, Jian;Yang, Bingzhuang;Liang, Xianwei

作者机构:

关键词: Buffalo;IVF;SCNT;Embryo;Developmental competence

期刊名称:BUFFALO BULLETIN ( 影响因子:0.172; 五年影响因子:0.234 )

ISSN: 0125-6726

年卷期: 2013 年 32 卷

页码:

收录情况: SCI

摘要: This study was conducted to investigate the developmental competence and blastocyst formation speed of three kinds of in vitro produced buffalo embryos: in vitro fertilized (IVF) embryos derived from oocytes collected either by aspirating of abattoir ovaries (abattoir-IVF) or by ovum pick up (OPU-IVF) matured in vitro, and somatic cell nuclear transferred (SCNT) embryos. The cleavage rate, blastocyst rate and blastocyst formation time of these embryos were recorded. The results showed that: (1) the cleavage rates of abattoir-IVF, OPU-IVF and SCNT embryos were not different form each other (52.5%, 63.4%, 56.4%, respectively), whereas the blastocyst rate of OPU-IVF embryos was higher than that of SCNT embryos (36.4% vs 19.8%, p<0.05); (2) as for abattoir ovaries-IVF embryos, the percentage of blastocysts harvested on Day 7 (46.8%) was higher than those harvested on Days 6, 8 and 9 (17.7%, 23.0%, 12.5%, respectively; p<0.01); as for OPU-IVF embryos, the percentage of blastocysts harvested on Day 7 (44.5%) was higher than those harvested on Days 6, 8 and 9 (17.6%, 27.8%, 9.7%, respectively; p<0.01); as for SCNT embryos, the percentages of blastocysts harvested on Day 6 and Day 7 (49.6% and 31.3%) was higher than those harvested on Days 5, 8 and 9 (7.4%, 8.5%, 1.7%, respectively; p<0.01). In conclusion, the developmental competence of SCNT embryos was poorer than that of OPU-IVF embryos, whereas the abattoir-IVF and OPU-IVF groups showed similar developmental competence. Furthermore, the blastocyst formation of SCNT embryos occurs earlier than the IVF embryos.

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