Optimization of Culture and Cryopreservation of Hand-Made Clone (HMC) Buffalo (Bubalus bubalis) Embryos

文献类型： 外文期刊

第一作者： Yang, Chunyan

作者： Yang, Chunyan;Pang, Chunying;Zheng, Haiying;Huang, Fenxiang;Wang, Jian;Shang, Jianghua;Yang, Bingzhuang;Liang, Xianwei

作者机构：

关键词： buffalo;hand-made clone;in vitro culture;cryopreservation

期刊名称：BUFFALO BULLETIN （ 影响因子：0.172； 五年影响因子：0.234 ）

ISSN： 0125-6726

年卷期： 2013 年 32 卷

页码：

收录情况： SCI

摘要： This study was conducted to optimize the culture and cryopreservation conditions for buffalo hand-made clone (HMC) embryos. Microdrop (MD), well of the well (WOW), flat surface (FS) systems were adopted to culture HMC reconstructed embryos in vitro. The derived HMC blastocysts were vitrified either with 40% EG, 25% EG + 25% DMSO or 20% EG + 20% DMSO + 0.5 M sucrose. Furthermore, the efficiencies of HMC and traditional somatic cell nuclear transfer (SCNT) were also compared. The results showed that: (1) the cleavage rate of HMC embryos cultured in WOW was higher than those cultured on FS (70.5% vs 55.8%, p<0.05) and in MD (70.5% vs 50.0%, p<0.01); also, the blastocyst rate of HMC embryos derived from WOW system (40.0%) was higher than those derived from FS (19.8%) and MD (8.3%) systems (p<0.01); (2) the cryosurvival rate of blastocysts vitrified with 20% EG + 20% DMSO + 0.5 M sucrose was higher than those vitrified with 40% EG (90.9% vs 62.0%, p<0.01); (3) both the fusion and blastocyst rates of HMC reconstructed embryos were higher than those of SCNT reconstructed embryos (89.1% vs 79.8%, p<0.05; 40.0% vs 19.8%, p<0.01), whereas the cryotolerances of HMC and SCNT blastocysts were not different from each other (90.9% vs 92.3%). In conclusion, WOW is the most suitable method for culture of HMC embryos, and vitrification of HMC blastocysts with 20% EG + 20% DMSO + 0.5M sucrose results in a high cryosurvival rate. HMC can be an alternative to traditional SCNT in buffalo.

分类号：

  • 相关文献

[1]Relationships between endogenous hormonal content and direct somatic embryogenesis in Watermelon (Citrullus lanatus) cotyledons. Zhang, Hui Jun,Wang, Qing,Wang, Qing. 2015

[2]Cell division and cell enlargement during potato tuber formation. Xu, X,Vreugdenhil, D,van Lammeren, AAM. 1998

[3]Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues. Hu, Pengfei,Pu, Yabin,Li, Xiayun,Guan, Weijun,Ma, Yuehui,Hu, Pengfei,Zhu, Zhiqiang,zhao, Yuhua.

[4]In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium. Peng, Xiayu,Wang, Liqin,Tong, Chen,Guo, Zhiqin,Yang, Mei,Peng, Xiayu. 2010

[5]Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy. Hu, G. J.,Hong, N.,Wang, L. P.,Wang, G. P.,Hong, N.,Wang, G. P.,Hu, H. J.. 2012

[6]Generation of peanut mutants by fast neutron irradiation combined with in vitro culture. Wang, Jing-Shan,Sui, Jiong-Ming,Qiao, Li-Xian,Xie, Yong-Dun,Guo, Hui-Jun,Liu, Lu-Xiang,Zhao, Li-Lan,Yu, Shan-Lin. 2015

[7]Effects of wheat dried distillers' grains with solubles and cinnamaldehyde on in vitro fermentation and protein degradation using the Rusitec technique. Li, Yangling,He, Maolong,Li, Chun,Forster, Robert,Beauchemin, Karen Anne,Yang, Wenzhu,Li, Yangling,Li, Chun.

[8]Derivation and Characteristics of ES-Like Cells in Chicken Fetal Ovarian. Zhang, Chuan-Sheng,Geng, Li-Ying,Yin, Chun-Guang,Li-Xin, D. U.. 2011

[9]Culture of Chicken Embryonic Stem Cells in Buffalo Rat Liver 3A Cells (BRL-3A) Conditioned Medium. Zhang, Chuan-Sheng,Geng, Li-Ying,Du, Li-Xin. 2011

[10]The Influence of Endogenous Hormones on Somatic Embryos Formation from Anthers of Hevea Brasiliensis Muell. Arg. Huijun, Z.,Xianfeng, Y.,Huasun, H.,Tiandai, H.,Quannan, Z.. 2016

[11]Main Factors Influencing the Efficient Propagation of Virulent or Attenuated Strains of Japanese encephalitis virus in BHK-21 Cells. Teng, Man,Luo, Jun,Xing, Guang-Xu,Cheng, Na,Yang, Yan-Yan,Deng, Rui-Guang,Zhang, Gai-Ping,Xing, Guang-Xu. 2012

[12]In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles. Wang, Hui,Li, Jun,Guo, Baoping,Wen, Hao,Zhang, Wenbao,Zhao, Li,Zhang, Zhuangzhi,McManus, Donald P.. 2016

[13]Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Hu, Guojun,Dong, Yafeng,Zhang, Zunping,Fan, Xudong,Ren, Fang,Zhou, Jun.

[14]Sweetpotato viruses in China. Wang, Qingmei,Zhang, Liming,Wang, Biao,Yin, Zhenfang,Feng, Chaohong,Wang, Qiaochun.

[15]Continuous in vitro cultivation of a recently identified Babesia that infects small ruminants in China. Guan, Guiquan,Ma, Miling,Liu, Aihong,Du, Pengfei,Ren, Qiaoyun,Li, Youquan,Wang, Jinming,Liu, Zhijie,Yin, Hong,Luo, Jianxun. 2012

[16]The Impact of Linolenic Acid on in vitro Development of Buffalo Embryos. Zheng, Haiying,Yang, Chunyan,Shang, Jianghua,Pang, Chunying,Huang, Fenxiang,Wang, Jian,Yang, Bingzhuang,Liang, Xianwei. 2013

[17]Repeated Ovum Pick-Up and In-vitro Embryo Production in Buffalo. Liang, X.,Zhang, X.,Yang, B.,Chen, M.,Huang, F.,Pang, C.,Liao, C.,Qin, G.,Wei, S.,Yang, C.. 2007

[18]Milk fat globule is an alternative to mammary epithelial cells for gene expression analysis in buffalo. Chen, Qiuming,Wu, Yanjun,Zhang, Mingyuan,Xu, Wenwen,Guo, Xiaoping,Yan, Xueyu,Deng, Haiying,Jiang, Qinyang,Yang, Xiurong,Lan, Ganqiu,Guo, Yafen,Jiang, Hesheng,Qin, Guangsheng.

[19]A field study on artificial insemination of swamp and crossbred buffaloes with sexed semen from river buffaloes. Lu, Yangqing,Liao, Yanqiong,Zhang, Ming,Yang, Xiaogan,Lu, Shengsheng,Xu, Huiyan,Lu, Kehuan,Liao, Yanqiong,Yang, Bingzhuang,Liang, Xianwei,Wu, Zhuyue,Liang, Yunbin.

[20]Four novel polymorphisms of buffalo INSIG2 gene are associated with milk production traits in Chinese buffaloes. Deng, Tingxian,Pang, Chunying,Ma, Xiaoya,Lu, Xingrong,Duan, Anqin,Zhu, Peng,Liang, Xianwei.

作者其他论文 更多>>

微信小程序