The Impact of Linolenic Acid on in vitro Development of Buffalo Embryos
文献类型: 外文期刊
第一作者: Zheng, Haiying
作者: Zheng, Haiying;Yang, Chunyan;Shang, Jianghua;Pang, Chunying;Huang, Fenxiang;Wang, Jian;Yang, Bingzhuang;Liang, Xianwei
作者机构:
关键词: buffalo;linolenic acid;embryo development
期刊名称:BUFFALO BULLETIN ( 影响因子:0.172; 五年影响因子:0.234 )
ISSN: 0125-6726
年卷期: 2013 年 32 卷
页码:
收录情况: SCI
摘要: The present study investigated the effect of linolenic acid supplementation on the early development of buffalo embryos in vitro. The presumptive zygotes were transferred to the vitro culture medium (TCM199, 10% FBS) supplemented with 0 (control), 10, 50, 100 and 200 mu M linolenic acid. Cleavage and blastocyst development rates were recorded on Day 2 and Day 6 to 9 after insemination, respectively. A total of 1482 COCs were used in ten independent replicates. The results showed that the cleavage rates in the groups of 0 (control), 10, 50, 100 and 200 mu M linolenic acid were 59.0 +/- 7.2%, 62.7 +/- 6.3%, 63.5 +/- 6.1%, 58.1 +/- 7.9% and 60.7 +/- 6.7%, respectively. No statistical difference was observed regarding cleavage rates among treatments (p>0.05). The treatment of COCs with 50 mu M linolenic acid resulted in a significantly higher percentage of blastocyst development rate compared to the control group and those supplemental with 10, 100 or 200 mu M linolenic acid (34.5 +/- 4.1% vs 25.4 +/- 8.2%, 28.85 +/- 7.59%, 26.41 +/- 9.51%, 29.45 +/- 10.60%, respectively, p<0.05). In contrast, no significant differences were found between the control group and the other treatments. In conclusion, the results of this study indicated that supplementation of linolenic acid in the vitro culture medium could enhance the blastocyst development in buffalo species and the optimal concentration of linolenic acid in the present procedures was 50 mu M.
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