Effects of Curcumin on Buffalo Embryonic Development In Vitro

文献类型： 外文期刊

第一作者： Shang, Jiang-Hua

作者： Shang, Jiang-Hua;Yang, Chun-Yan;Zheng, Hai-Ying;Jing-Qin;Wang, Jian;Huang, Fen-Xiang;Yang, Bing-Zhuang;Liang, Xiang-Wei

作者机构：

关键词： buffalo;curcumin;embryo;in vitro culture (IVC)

期刊名称：BUFFALO BULLETIN （ 影响因子：0.172； 五年影响因子：0.234 ）

ISSN： 0125-6726

年卷期： 2013 年 32 卷

页码：

收录情况： SCI

摘要： The present study investigated the effects of curcumin on buffalo embryonic development by incubating zygotes without or with the compound during in vitro culture (IVC). Cumulus-oocyte complexes retrieved from antral follicles of water buffalo ovaries were matured for 20-22 hrs and inseminated with motile buffalo sperm in Tyrode's medium for 24 hrs. The presumed zygotes were washed 3 times and transferred into 50 mu l droplets of IVC medium (TCM 199 + 10% FBS) supplemented with curcumin in various concentrations (0 mu M, 2.5 mu M, 5 mu M, 10 mu M and 20 mu M, respectively) and co-cultured with buffalo cumulus cells monolayer for more than 7 days to evaluate the developmental ability of embryos (0, 2.5, 5, 10 and 20 mu M groups, respectively). The cleavage rate (CR) and blastocyst rate (BR) were assessed at 48 hrs and 168 hrs, respectively, after fertilization (0 hrs). The results showed that there was a slight decrease in the CRs from 0 mu M group (control) to 20 mu M group (60.34%, 56.18%, 57.69%, 55.09% and 45.58%, respectively) but there was no statistical difference among all groups. However, the BR in 20 mu M group (9.82%) was significantly lower (p<0.05) than those from 0 mu M to 10 mu M groups (30.61%, 26.30%, 26.80% and 27.48%, respectively). Thus, we concluded that exposure of buffalo zygotes with high-dose curcumin (20 mu M) during IVC has injurious effects on embryonic development in vitro.

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