Compare of Phenotypic Variation with the Polymorphism of One Coagulation Related Gene Locus in Buffalo Kappa-Casein

文献类型： 外文期刊

第一作者： Lin, Bo

作者： Lin, Bo;Yang, Bingzhuang;Li, Ling;Yan, Tang;Long, Haoru;Ren, Daxi;Zeng, Qingkun

作者机构：

关键词： buffalo, kappa-casein;gene polymorphism;phenotypic variation;relationship

期刊名称：BUFFALO BULLETIN （ 影响因子：0.172； 五年影响因子：0.234 ）

ISSN： 0125-6726

年卷期： 2013 年 32 卷

页码：

收录情况： SCI

摘要： Kappa-casein (kappa-CN) is a protein which highly correlated with coagulation property of dairy cow milk, while its function was influenced by its genetic polymorphism. The phenotypic variation of kappa-casein from buffalo was analysized by High Performance Liquid Chromatography (HPLC), and genetic polymorphism of kappa-casein was also determined the by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in this study, aimed to research the relationship between buffalo kappa-CN genetic polymorphism and its phenotypic variation. The milk proteins from 98 individuals of water buffalo without consider their breed were analyzed by HPLC, then blood DNA was extracted to determine polymorphism of one polymorphic locus in kappa-CN gene which generally been recognized to related with milk coagulation property. The results showed three obvious kappa-CN phenotypes can be screened by HPLC, the frequencies of BB, BC and AB were 76.5%, 6.1% and 17.3%, respectively. Three genotype, AA, BB and AB were also screened from the researched kappa-CN gene polymorphic locus, frequencies of each genotype were 4.1%, 26.5% and 69.4%, respectively, and the allele frequencies of A and B were 17.3% and 82.7%. Compare the genetic polymorphism of kappa-CN with its phenotypic variation showed there were no corresponding relationships between kappa-CN genotypes with its phenotypes, all the three genotypes can be found in BB phenotypic samples, while they also can be found in AB phenotypic samples. The results indicated the researched polymorphic locus of kappa-CN gene in this study would not the main locus influencing phenotype of buffalo kappa-CN, suggested effect of this locus on milk coagulation would be exerted by influencing other characteristics of kappa-CN while not HPLC detectable variation.

分类号：

  • 相关文献

[1]Phenotypic and Genotypic Characterization of Phytophthora infestans Isolates from China. Li, Benjin,Chen, Qinghe,Lv, Xin,Lan, Chengzhong,Zhao, Jian,Qiu, Rongzhou,Weng, Qiyong.

[2]Genetic diversity within Oryza rufipogon germplasms preserved in Chinese field gene banks of wild rice as revealed by microsatellite markers. Zhang, Chi-Hong,Li, Dao-Yuan,Pan, Da-Jian,Jia, Ji-Zeng,Dong, Yu-Shen.

[3]Molecular characterization of two candidate genes associated with coat color in Tibetan sheep (Ovis arise). Han Ji-long,Guo Ting-ting,Yue Yao-jing,Liu Jian-bin,Niu Chun-e,Yang Bo-hui,Yang Min,Wang Chao-feng. 2015

[4]Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats. Chu, M. X.,Fang, L.,Di, R.,Cao, G. L.,Feng, T.,Peng, Z. L.,Chen, H. Q.,Zhang, Y. J.,Li, N.. 2012

[5]THE PHYLOGENETIC RELATIONSHIPS AMONG GERMPLASM RESOURCES OF WILD RAMIE (BOEHMERIA NIVEA L. GAUD) IN CHINA BASED ON trnL-F AND ITS SEQUENCES. Yu Runqing,Baloch, Sana Ullah,Liu Lijun,Zhong Xinyue,Wang Bo,Peng Dingxiang,Pan Qihui,Gong Songlin. 2015

[6]Genetic diversity and relationships among 47 loquat varieties revealed by EST-SSR markers. Li, Xiao-ying,Xu, Hong-xia,Chen, Jun-wei. 2013

[7]Vertical-Flow Constructed Wetlands Applied in a Recirculating Aquaculture System for Channel Catfish Culture: Effects on Water Quality and Zooplankton. Zhang, Shi-yang,Zhou, Qiao-hong,Xu, Dong,He, Feng,Cheng, Shui-ping,Liang, Wei,Du, Cheng,Wu, Zhen-bin,Zhang, Shi-yang,Du, Cheng,Zhang, Shi-yang. 2010

[8]Spatial and seasonal variations in the planktonic ciliate community and its relationship with environmental factors in Daya Bay, the South China Sea. Wu, Fengxia,Qi, Zhanhui,Huang, Honghui,Wu, Fengxia,Qi, Zhanhui,Huang, Honghui,Wu, Fengxia,Qi, Zhanhui,Huang, Honghui,Huang, Jianrong. 2017

[9]Relationship between white spot syndrome virus (WSSV) loads and characterizations of water quality in Litopenaeus vannamei culture ponds during the tropical storm. Zhang, J. S.,Li, Z. J.,Wen, G. L.,Luo, L.,Dong, H. B.,Wang, G. L.,Zhang, H. J.,Luo, L.. 2016

[10]Levels of Cu, Mn, Fe and Zn in Cow Serum and Cow Milk: Relationship with Trace Elements Contents and Chemical Composition in Milk. Wang, Hui,Liu, Yongming,Qi, Zhiming,Wang, Shengyi,Liu, Shixiang,Dong, Shuwei,Xia, Xinchao,Li, Shengkun,Liu, Zhiqi. 2014

[11]Genetic diversity and relationship of clonal tea (Camellia sinensis) cultivars in China as revealed by SSR markers. Fang, W.,Cheng, H.,Duan, Y.,Jiang, X.,Li, X.,Cheng, H.. 2012

[12]Estimating Grain Yield Based on BSW and SPAD at Grain Filling Stage in Double Rice Cropping System of China. Liu Kailou,Zhang Huimin,Liu Kailou,Li Yazhen,Yu Paolan,Yan, Wu,Zhou Lijun,Hu Huiwen. 2016

[13]Influences of season, parity, lactation, udder area, milk yield, and clinical symptoms on intramammary infection in dairy cows. Zhang, Z.,Li, X. P.,Yang, F.,Luo, J. Y.,Wang, X. R.,Liu, L. H.,Li, H. S..

[14]An Improved Protocol Suitable for Polymorphism Studies in Chinese Jujube (Ziziphus jujuba Mill.). Bai, R. X.,Peng, J. Y.,Li, L.,Zhang, Y.,Han, B.,Zhang, L. S.,Li, X. Y.. 2009

[15]RAPD study on inter-species relationships in Roegneria (Poaceae : Triticeae). Zhou, YH,Yang, JL,Zheng, YL,Yan, J,Jia, JZ,Wei, YM. 1999

[16]A Functional Antagonistic Relationship between Auxin and Mitochondrial Retrograde Signaling Regulates Alternative Oxidase1a Expression in Arabidopsis. Ivanova, Aneta,Law, Simon R.,Duncan, Owen,Zhang, Botao,Radomiljac, Jordan D.,Whelan, James,Ivanova, Aneta,Narsai, Reena,Lee, Jae-Hoon,Van Aken, Olivier,Radomiljac, Jordan D.,van der Merwe, Margaretha,Yi, KeKe.

[17]Investigation of the Spectroscopic Information on Functional Groups Related to Carbohydrates in Different Morphological Fractions of Corn Stover and Their Relationship to Nutrient Supply and Biodegradation Characteristics. Xin, Hangshu,Ding, Xue,Zhang, Liyang,Wang, Xiaofan,Zhang, Yonggen,Ding, Xue,Sun, Fang.

[18]QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Fu-Ding Sun,Jian-Hong Zhang,Shu-Fang Wang,Wan-Kui Gong,Yu-Zhen Shi,Ai-Ying Liu,Jun-Wen Li,Ju-Wu Gong,Hai-Hong Shang,You-Lu Yuan.

[19]Molecular mapping of quantitative trait loci for kernel morphology traits in a non-1BL.1RS x 1BL.1RS wheat cross. Yonggui Xiao,Shengmei He,Jun Yan,Yong Zhang,Yelun Zhang,Yunpeng Wu,Xianchun Xia,Jichun Tian,Wanquan Ji,Zhonghu He.

[20]Phenotypic Variation and Genetic Diversity in the Collections of Erianthus Arundinaceus (Retz.) Jesw. Li, Yang-Rui,Liu, Xi-Hui,Song, Huan-Zhong,Zhang, Ge-Min,Duan, Wei-Xing,Zhang, Rong-Hua,Li, Yang-Rui. 2017

作者其他论文 更多>>

微信小程序