Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

文献类型： 外文期刊

第一作者： Zhang, Xuan

作者： Zhang, Xuan;Chu, Yue-feng;Zhao, Ping;Gao, Peng-cheng;He, Ying;Wang, Nu;Lu, Zhong-xin

作者机构：

关键词： Antigenicity;EF-Tu;Mycoplasma ovipneumoniae

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）

ISSN： 0253-8318

年卷期： 2013 年 33 卷 4 期

页码：

收录情况： SCI

摘要： Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E. coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease. (C) 2013 PVJ. All rights reserved

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