Solexa Sequencing of MicroRNAs on Chromium Metabolism in Broiler Chicks

文献类型: 外文期刊

第一作者: Pan, Y. Z.

作者: Pan, Y. Z.;Wu, S. G.;Zhang, H. J.;Yue, H. Y.;Qi, G. H.;Pan, Y. Z.;Dai, H. C.

作者机构:

关键词: Chromium picolinate;MicroRNAs;Broilers;Solexa sequencing;Real-time qPCR

期刊名称:JOURNAL OF NUTRIGENETICS AND NUTRIGENOMICS ( 影响因子:2.125; 五年影响因子:2.286 )

ISSN: 1661-6499

年卷期: 2013 年 6 卷 3 期

页码:

收录情况: SCI

摘要: Aim: The aim of this study was to determine the effect of chromium picolinate (CrPic) on the differential expression of the known microRNAs (miRNAs) in broiler skeletal muscle. Methods and Results: A total of 288 1-day-old male Arbor Acres broilers were randomly assigned to one of four dietary treatments supplemented with 0, 0.4, 2.0, or 10.0 mg.kg(-1) CrPic, respectively. Dietary CrPic supplementation at 10.0 mg.kg(-1) increased the average daily feed intake in broilers (p < 0.05). On day 42, the serum total protein level was highest in animals treated with 2.0 mg.kg(-1) (p < 0.05) and 10.0 mg.kg(-1) CrPic (p < 0.05). Dietary supplementation with 10.0 mg.kg(-1) CrPic decreased the levels of serum glucose (p < 0.05) on day 42 and of serum triglyceride (p < 0.05) on days 21 and 42. To further identify miRNAs from broiler skeletal muscles, we sequenced two small RNA libraries using the Solexa sequencing approach, and 57 miRNAs were found to be significantly differentially expressed (p < 0.05). Among them, 6 upregulated and 2 downregulated miRNAs were validated by real-time qPCR (p < 0.05). Conclusions:The results of the present study provide a valuable clue regarding the role of miRNA target genes in the mechanism of the dietary CrPic effect on protein synthesis in skeletal muscles of broilers. (C) 2013 S. Karger AG, Basel

分类号:

  • 相关文献

[1]Effects of Chromium Picolinate on Oxidative Damage in Primary Piglet Hepatocytes. Tan, Gao-Yi,Bi, Jin-Ming,Zhang, Min-Hong,Feng, Jing-Hai,Xie, Peng,Zheng, Shan-Shan. 2008

[2]Study of Oxidative Damage in Growing-Finishing Pigs with Continuous Excess Dietary Chromium Picolinate Intake. Tan, Gao-Yi,Zheng, Shan-Shan,Zhang, Min-Hong,Feng, Jing-Hai,Xie, Peng,Bi, Jin-Ming. 2008

[3]Study on changes and mechanisms of cytokines for alloxan-induced hepatic injury by Cr3+-treatment in mice. Fan, Weijiang,Fan, Weijiang,Wang, Wenhang,Liu, Anjun,Zhang, Lan,Qi, Lihong.

[4]Regulation of organic nucleic acids and serum biochemistry parameters by dietary chromium picolinate supplementation in swine model. Yang Jiajun,Han Aiyun,Zheng Shanshan,Zhang Minhong. 2011

[5]Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp HF-1: nicotine degradation and microbial community analysis. Wang, Xin,Tang, Lu,Wang, Haixia,Min, Hang,Lu, Zhenmei,Yao, Yanlai. 2013

[6]Isolation and Expression Analysis of Catalase Genes in Erianthus arundinaceus and Sugarcane. Liu, Yang,Hu, Xiaowen,Yao, Yanli,Xu, Lei,Xing, Shulian. 2016

[7]Molecular Cloning and Functional Analysis of an Ethylene Receptor Gene from Sugarcane (Saccharum spp.) by Hormone and Environmental Stresses. Yang, Li-Tao,Li, Yang-Rui,Wang, Ai-Qin,Yang, Li-Tao,Li, Yang-Rui,Wang, Ai-Qin,Ye, Xing-Zhi,Huang, Jing-Li,Niu, Jun-Qi,Liu, Ming,Yang, Li-Tao,Li, Yang-Rui,Pan, Yong-Bao. 2015

[8]Effects of two different forage sources on mammary gland growth, mammary cell turnover and activity in early lactation dairy cows. Qin, Tong,Wang, Haoyu,Hao, Haisheng,Wang, Dong,Zhu, Huabin,Bu, Dengpan.

[9]Molecular characterization of StpkaC2 and expression patterns of both PKA-c isoforms during the invasive growth of Setosphaeria turcica. Hao, Zhimin,Tong, Yameng,Han, Yue,Wu, Di,Yang, Zheng,Shen, Shen,Gong, Xiaodong,Cao, Zhiyan,Gu, Shouqin,Dong, Jingao,Li, Zhiyong.

[10]Genome-wide identification of Thellungiella salsuginea microRNAs with putative roles in the salt stress response. Zhang, Quan,Sun, Wei,Liu, Yan,Wang, Xingjun,Zhao, Yanxiu,Zhao, Chuanzhi,Li, Ming,Xia, Han,Sun, Mingnan,Li, Aiqin,Li, Changsheng,Zhao, Shuzhen,Hou, Lei,Picimbon, Jean-Francois,Wang, Xingjun. 2013

[11]Analysis of differentially expressed genes in response to endogenous cytokinins during cotton leaf senescence. P. ZHAO,N. ZHANG,Z.J. YIN,Y.D. LIU,F.F. SHEN. 2013

[12]Transcriptome of High-Sucrose Sugarcane Variety GT35. Gao, Yi-Jing,Gui, Yi-Yun,Chen, Zhong-Liang,Qin, Cui-Xian,Wang, Miao,Li, Yang-Rui,Liao, Qing,Li, Yang-Rui,Yang, Li-Tao. 2016

[13]Identification and characterization of microRNAs in the ovaries of multiple and uniparous goats (Capra hircus) during follicular phase. Ling, Ying-Hui,Ren, Chun-Huan,Guo, Xiao-Fei,Huang, Ya-Feng,Luo, Jian-Chuan,Zhang, Yun-Hai,Zhang, Xiao-Rong,Zhang, Zi-Jun,Ling, Ying-Hui,Ren, Chun-Huan,Guo, Xiao-Fei,Huang, Ya-Feng,Luo, Jian-Chuan,Zhang, Yun-Hai,Zhang, Xiao-Rong,Zhang, Zi-Jun,Xu, Li-Na. 2014

[14]Successful detection of foreign inserts in transgenic rice TT51-1 (BT63) by RNA-sequencing combined with PCR. Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Huang, Shunmou,Wu, Gang,Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Wu, Gang.

[15]Characterization and profiling of MicroRNAs in posterior silk gland of the silkworm (Bombyx mori). Song, Fei,Wang, Xin,Chen, Chen,Fan, Yangyang,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia,Tang, Shunming,Huang, Jinshan,Guo, Xijie,Shen, Xingjia.

[16]Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells. Shen, Binglei,Zhang, Liying,Lu, Chunyan,Zhang, Yonghong,Yang, Runjun,Zhao, Zhihui,Shen, Binglei,Pan, Qiqi,Lian, Chuanjiang,Lian, Chuanjiang. 2016

[17]Solexa sequencing based transcriptome analysis of Helicoverpa armigera larvae. Li, Jigang,Li, Xiumin,Chen, Yongli,Yang, Zhongxiang,Guo, Sandui.

[18]Transcriptome profile and differentially expressed genes analysis in winter wheat under cold stress conditions. Xie, Dongwei,Li, Zhuofu,Xie, Dongwei. 2015

[19]Comparison of skeletal muscle miRNA and mRNA profiles among three pig breeds. Hou, Xinhua,Yang, Yalan,Zhu, Shiyun,Hua, Chaoju,Zhou, Rong,Mu, Yulian,Tang, Zhonglin,Li, Kui,Yang, Yalan,Tang, Zhonglin.

[20]Identification of microRNAs from Amur grape (vitis amurensis Rupr.) by deep sequencing and analysis of microRNA variations with bioinformatics. Wang, Chen,Han, Jian,Kibet, Korir Nicholas,Kayesh, Emrul,Shangguan, Lingfei,Li, Xiaoying,Fang, Jinggui,Liu, Chonghuai. 2012

作者其他论文 更多>>