CLONING OF BASIC FIBROBLAST GROWTH FACTOR FROM CHINESE SMALL TAIL HAN SHEEP AND ITS EFFECTS ON PROLIFERATION OF MURINE C2C12 MYOBLASTS

文献类型： 外文期刊

第一作者： Liu, Hua-zhong

作者： Liu, Hua-zhong;Chen, Shao-hong;Luo, Ping;Shang, Jiang-hua

作者机构：

关键词： bFGF;cloning;C2C12;MEK/ERK;Small Tail Han sheep

期刊名称：SLOVENIAN VETERINARY RESEARCH （ 影响因子：0.749； 五年影响因子：0.558 ）

ISSN： 1580-4003

年卷期： 2013 年 50 卷 1 期

页码：

收录情况： SCI

摘要： To further understand characteristics and functions of basic fibroblast growth factor (bFGF, FGF-2) as well as its effects on skeletal muscle, a coding sequence of 18kDa-bFGF gene was cloned from Chinese Small Tail Han sheep, an excellent indigenous animal breed in China. The DNA sequence and deduced protein sequence were analyzed and aligned with the counterparts of other published animals. The results indicated that most hallmarks of the newly cloned bFGF gene sequence were found to be similar to those of published species, which implied further that bFGF genes have been conservative in evolution. The complete coding sequence for the gene was inserted into pET-28a plasmid and expressed predicted fusion protein in E. coli. The product was soluble and possessed of biological activity, it is sure to be a good source for further investigation. In addition, effects of the products on proliferation of murine C2C12 myoblasts were investigated. The findings demonstrated that the ovine recombinant bFGF accelerated in vitro the proliferation of murine C2C12 myoblasts, and MEK/ERK-MAPK signaling pathway participated in the regulative mechanism.

分类号：

  • 相关文献

[1]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[2]bFGF signaling-mediated reprogramming of porcine primordial germ cells. Zhang, Yu,Ma, Jing,Lv, Jiawei,Wei, Renyue,Cong, Yimei,Liu, Zhonghua,Li, Hai,Cong, Yimei.

[3]Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation. Chao, Zhe,Zheng, Xin-Li,Sun, Rui-Ping,Liu, Hai-Long,Huang, Li-Li,Cao, Zong-Xi,Wang, Feng,Deng, Chang-Yan.

[4]Candidate genes with ovulation by differential display PCR in Small Tail Han sheep. Liu Shufang,Li Hongbin,Song Xuemei,Wang Aihua,Wei Caihong,Du Lixin. 2006

[5]Dynamic comparison of genetic diversity in a Small Tail Han sheep population using meta-analysis. E, G. X.,Huang, Y. F.,Zhao, Y. J.,Qiu, X. Y.,Chen, L. P.,He, J. N.,Liu, N.,Zhong, T.,Ma, Y. H.. 2015

[6]Polymorphism of Prophet of Pit-1 gene and its relationship with litter size of Small Tail Han sheep. Liu, Qiuyue,Chu, Mingxing,Di, Ran,Feng, Tao,Geng, Caixia,Chen, Hongquan,Jin, Mei,Zhang, Yingjie,Li, Ning. 2015

[7]Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle. Li, Z.,Huang, H.,Zhao, H.,Meng, K.,Shi, P.,Yang, P.,Luo, H.,Wang, Y.,Yao, B.,Li, Z.,Zhao, J..

[8]Cloning and Expression Analysis of Eight Upland Cotton Pentatricopeptide Repeat Family Genes. Han, Zongfu,Kong, Fanjin,Deng, Yongsheng,Wang, Zongwen,Shen, Guifang,Wang, Jinghui,Duan, Bing,Li, Ruzhong,Qin, Yuxiang.

[9]Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Pan, Hongyu,Wei, Yi,Xin, Furong,Zhou, Mingguo,Zhang, Shihong.

[10]Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage. Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Zhang, Dianchang,Huang, Jianhua,Guo, Yihui. 2008

[11]Cloning, expression and functional analysis of a delta 6-desaturase gene from the silkworm, Bombyx mori L.. Yu, Hai-Yan,Zhou, Zhi-Feng,Jia, Jun-Qiang,Gui, Zhong-Zheng,Jia, Jun-Qiang,Gui, Zhong-Zheng. 2016

[12]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[13]Cloning and sequence analysis of an actin gene in aloe. He, D. W.,Li, J.,Wen, G. Q.,Liu, X. H.,Liao, C. M.. 2014

[14]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[15]Cloning and expression analysis of SPL8 homolog from pak choi (Brassica rapa subsp chinensis). Zhang, Jing,Ping, A-Min,Wang, Xue-Ting,Li, Gai-Zhen,Li, Mei-Lan,Li, Gai-Zhen,Zhu, Zhu-Jun,Xing, Guo-Ming,Hou, Lei-Ping. 2017

[16]Cloning, expression of a peroxiredoxin gene from Acinetobacter sp SM04 and characterization of its recombinant protein for zearalenone detoxification. Yu, Yuanshan,Wu, Hui,Yu, Yuanshan,Wu, Hui,Tang, Yuqian,Qiu, Liping. 2012

[17]Na+/K+-ATPase alpha-subunit in swimming crab Portunus trituberculatus: molecular cloning, characterization, and expression under low salinity stress. Han, Xiaolin,Liu, Ping,Gao, Baoquan,Wang, Haofeng,Duan, Yafei,Xu, Wenfei,Chen, Ping,Han, Xiaolin. 2015

[18]Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum. Zhang Fan,Wang Zhong,Yang Qing,Huang Quan-sheng,Huang Le-ping. 2010

[19]Cloning and Characterization of Goose PPAR gamma Gene Comparison with Other Vertebrates. Wu, H. L.,Kong, F. L.,Qiu, M. H.,Zhou, B.,Luo, J.,Zhu, Q.,Wang, H. Y.,Liu, Y.,He, D. Q.. 2012

[20]Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3. Liu, X.,Wang, L. G.,Zhang, L. C.,Yan, H.,Zhao, K. B.,Liang, J.,Li, N.,Pu, L.,Zhang, T.,Wang, L. X.. 2016

作者其他论文 更多>>

微信小程序