Identification and purification of an antibiotic protein produced by Pseudomonas fluorescens strain CZ with inhibitory effect to tobacco mosaic virus

文献类型： 外文期刊

第一作者： Shen, Lili

作者： Shen, Lili;Wang, Fenglong;Sun, Huiqing;Qian, Yumei;Yang, Jinguang;Dong, Xiaowei;Ma, Qiang

作者机构：

关键词： Nicotiana tabacum;Pseudomonas fluorescens;Tobacco mosaic virus

期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）

ISSN： 0261-2194

年卷期： 2012 年 42 卷

页码：

收录情况： SCI

摘要： Tobacco mosaic virus (TMV) is a broad host range virus that inhibits the growth of a wide range of plant species, with devastating effects on crop production. In this study, the inhibitory effect of Pseudomonas fluorescens strain CZ against TMV was analyzed. Inoculation of a necrotic spot host, Nicotiana tabacum cv. Samsum NN, showed that strain CZ was most effective (99.5% suppression in vitro) when applied simultaneously with TMV, and was slightly less effective following preventive application (95.8%). Post-infection control was shown to confer the lowest inhibitory effect (69.7%) against TMV. The crude extracellular protein at concentrations of 5.0724 mu g/ml and 0.9867 mu g/ml conferred the suppression in vitro of 100% and 80.24%, respectively. Crude protein was extracted by salting out using ammonium sulfate from the CZ culture and purified by anion exchange chromatography, sephadex G-75 gel chromatography and SDS-polyacrylamide gel electrophoresis. The molecular weight of the protein was 39.4 kDa and it conferred 74.81% inhibition against TMV. (C) 2012 Elsevier Ltd. All rights reserved.

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