Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both In Vitro and In Vivo

文献类型： 外文期刊

第一作者： Guo, Xiaoyu

作者： Guo, Xiaoyu;Jia, Hong;Yuan, Weifeng;Hou, Shaohua;Zhu, Hongfei;Guo, Xiaoyu;Manuel Sanchez-Vizcaino, Jose;Guo, Xiaoyu;Manuel Sanchez-Vizcaino, Jose;Zhang, Quan;Sun, Yao;Zhu, Gang

作者机构：

关键词： antibody titer;CpG motif;FMD killed vaccine;lymphocyte transformation test;stimulation Index

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2012 年 74 卷 12 期

页码：

收录情况： SCI

摘要： A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed higher Stimulation Index (SI) compared to the positive control (LPS). In a following animal experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.

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