Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro

文献类型： 外文期刊

第一作者： Li Xiangchen

作者： Li Xiangchen;Yao Yaxin;Wang Yipeng;Li Heping;Guan Weijun;Ma Yuehui

作者机构：

关键词： Bovine;Embryos;Oocyte;Vitrification

期刊名称：INDIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：0.316； 五年影响因子：0.366 ）

ISSN： 0367-8318

年卷期： 2012 年 82 卷 11 期

页码：

收录情况： SCI

摘要： The effectiveness of existing procedures based on viable embryos per oocyte frozen remains low compared to use of fresh oocytes in cattle, and the probable causes of the freezing-induced reduction in oocyte developmental competence include chromosome, cytoskeleton disruption, or even the choice of the frozen stage of the oocytes. In the present study, after vitrification, the survival rate of GV and MI oocytes were significantly lower than those in MII. The vitrifid GV oocyte showed some chromosomes dispersed and less condensed, The vitrifid MI oocytes with "Fading'' and discontinuous staining pattern were as having an abnormal microfilament distribution. The stage of GV and MI, both cleavage rate and blastocyst formation rate were reduced significantly. Therefore, the cryopreservation of GV or MI oocytes seems could not instead of the MII oocytes.

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