OPTIMIZATION OF FLASK CULTURE MEDIUM AND CONDITIONS FOR HYALURONIC ACID PRODUCTION BY A STREPTOCOCCUS EQUISIMILIS MUTANT NC2168

文献类型： 外文期刊

第一作者： Chen, Yong-Hao

作者： Chen, Yong-Hao;Chen, Yong-Hao;Liu, Li;Liu, Hong-Zhi;Wang, Qiang;Li, Jun

作者机构：

关键词： Streptococcus equisimilis mutant;hyaluronic acid;optimization;culture medium;culture conditions

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2012 年 43 卷 4 期

页码：

收录情况： SCI

摘要： A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116 by ultraviolet ray combined with C-60(o)-gamma ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal yield of HA could be obtained when the concentrations of yeast extract, peptone, glucose, and MgSO4 were set at 3 g/100 mL, 2 g/100 mL, 0.5 g/100 mL and 0.15 g/100 mL, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a remarkable increase in the output of HA and the maximum of the predicted HA production was 174.76 mg/L. The model developed was accurate and reliable for predicting the production of HA by NC2168. Cultivation conditions were optimized by an orthogonal experimental design and the optimal conditions were as follows: temperature 33 degrees C, pH 7.8, agitation speed 200 rpm, medium volume 20 mL.

分类号：

  • 相关文献

[1]Statistical Optimization of Culture Media and Conditions for Production of Mannan by Saccharomyces cerevisiae. Liu, Hong-Zhi,Wang, Qiang,Liu, Yuan-Yuan,Fang, Fang.

[2]The optimization of fermentation conditions and enzyme properties of Stenotrophomonas maltophilia for protease production. Wang, Zaigui,Sun, Linghong,Cheng, Jia,Liu, Chaoliang,Liu, Ying,Tang, Xiangfang,Zhang, Hongfu.

[3]Effects of components in culture medium on glutamate decarboxylase activity and gamma-aminobutyric acid accumulation in foxtail millet (Setaria italica L.) during germination. Bai, Qingyun,Gu, Zhenxin,Cao, Xiaohong,Li, Yan,Liu, Kunlun,Bai, Qingyun,Chai, Meiqing. 2009

[4]Effects of selective medium on lipid accumulation of chlorellas and screening of high lipid mutants through ultraviolet mutagenesis. Deng, Xiaodong,Li, Yajun,Fei, Xiaowen,Fei, Xiaowen. 2011

[5]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[6]Optimization of culture medium and temperature for the in vitro germination of oil palm pollen. Lin, Yiyun,Wang, Yong,Shi, Peng,Li, Jing,Yang, Yaodong,Lei, Xintao,Iqbal, Amjad.

[7]Effects of Different Oils on the Fatty Acid Profiles of Culture Medium and Ruminal Microorganisms in vitro. Wang, M. Z.,Wang, H. R.,Yu, L. H.,Bu, D. P.,Wang, J. Q.. 2012

[8]Ovules culture and plant formation of hybrid progeny of seedless grape. Ding, HF,Qi, GM.

[9]Optimization of culture conditions for violacein production by a new strain of Duganella sp B2. Jiang, Peixia,Lu, Yuan,Xing, Xin-Hui,Wang, Haisheng,Ruan, Zhiyong,Jiang, Ruibo,Lou, Kai,Wei, Dong. 2009

[10]Optimization of trans lutein from pumpkin (Cucurbita moschata) peel by ultrasound-assisted extraction. Song, Jiangfeng,Yang, Qiuming,Huang, Wuyang,Xiao, Yadong,Li, Dajing,Liu, Chunquan,Yang, Qiuming. 2018

[11]Evaluation and Screening of Potential Non-Food Biodiesel Plants from Native Wild Species of Northwestern China. Qin, Shuo,Xue, Shuai,Xie, Guang Hui,Qin, Shuo,Xie, Guang Hui,Qin, Shuo,Xue, Shuai,Steinberger, Yosef,Li, Gui Ying. 2015

[12]Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis of the volatile components of longan (Dimocarpus longan Lour.). Zhang, Yi,Gao, Bei,Zhang, Mingwei,Zhang, Yi,Gao, Bei,Zhang, Mingwei,Xu, Yujuan,Shi, John. 2009

[13]Optimization of extraction conditions of total phenol from lotus (Nelumbo nuficera Gaertn) rhizome. Yang, Dong-Mei,Zhang, Qian,Wang, Hui-Li,Zhu, Xing-Yi,Wang, Qiu-Shuang,Ying, Tie-Jin. 2012

[14]Optimization of postharvest ultrasonic treatment of strawberry fruit. Cao, Shifeng,Hu, Zhichao,Pang, Bin. 2010

[15]Isolation of flavonoids from mulberry (Morus alba L.) leaves with macroporous resins. Wang, Jun,Wu, Fu An,Zhao, Hui,Liu, Li,Wu, Qiu Sheng. 2008

[16]Optimization and test for structural parameters of UAV spraying rotary cup atomizer. Zhou Qingqing,Xue Xinyu,Qin Weicai,Cai Chen,Zhou Liangfu. 2017

[17]Optimization and physicochemical properties of nutritional protein isolate from pork liver with ultrasound-assisted alkaline extraction. Zou, Ye,Bian, Huan,Li, Pengpeng,Sun, Zhilan,Sun, Chong,Zhang, Muhan,Geng, Zhiming,Xu, Weimin,Wang, Daoying. 2018

[18]Combined effect of temperature, salinity and density on the growth and feed utilization of Nile tilapia juveniles (Oreochromis niloticus). Qiang Jun,Wang Haizhen,Wang Hui,Xu Pao,Li Ruiwei. 2012

[19]A novel Ganoderma lucidum G0119 fermentation strategy for enhanced triterpenes production by statistical process optimization and addition of oleic acid. Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung.

[20]Optimization of fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05. Wang, P.,Jiang, X.,Jiang, Y.,Hu, X.,Jiang, Y.,Wang, P.,Hu, X.,Hwang, H..

作者其他论文 更多>>

微信小程序