DEVELOPMENT AND CHARACTERIZATION OF 59 POLYMORPHIC CDNA-SSR MARKERS FOR THE EDIBLE OIL CROP SESAMUM INDICUM (PEDALIACEAE)

文献类型： 外文期刊

第一作者： Wang, Linhai

作者： Wang, Linhai;Zhang, Yanxin;Qi, Xiaoqiong;Gao, Yuan;Zhang, Xiurong;Wang, Linhai;Zhang, Yanxin;Qi, Xiaoqiong;Gao, Yuan;Zhang, Xiurong

作者机构：

关键词： cDNA-SSR;genetic structure;microsatellite;Pedaliaceae;Sesamum indicum

期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）

ISSN： 0002-9122

年卷期： 2012 年 99 卷 10 期

页码：

收录情况： SCI

摘要： Premise of the study: Polymorphic simple sequence repeat markers from transcript sequences (cDNA-simple sequence repeat [SSR]) were developed for the edible oil crop Sesamum indicum to facilitate the genetic study of this species. Methods and Results: We found 7702 SSR loci in the 60 960 unigenes, and 1550 primer pairs were designed and synthesized. In total, 59 primer pairs showed polymorphism within 36 individuals; the number of alleles per locus ranged from two to four, and the expected and observed heterozygosity ranged from 0.05 to 0.74 and 0 to 0.30, respectively. Conclusions: These polymorphic markers will greatly facilitate studies of the genetic structure of S. indicum populations as well as the identification and conservation of the species.

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