Identification and molecular characterization of a new beta-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae

文献类型： 外文期刊

第一作者： Peng, Huan

作者： Peng, Huan;Huang, Wenkun;Wang, Gaofeng;Peng, Deliang;Gao, Bingli;Moens, Maurice

作者机构：

关键词： Cellulase;Cell wall degradation;Parasitism gene;Plant-parasitic nematode

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2012 年 134 卷 2 期

页码：

收录情况： SCI

摘要： Secretory proteins encoded by genes expressed in the pharyngeal gland cells of plant-parasitic nematodes have key roles in nematode parasitism of plants. A new beta-1,4-endoglucanase gene (designated Ha-eng-1a) was isolated from the cereal cyst nematode Heterodera avenae. The cDNA of Ha-eng-1a encoded a deduced 463-amino acid sequence containing a catalytic domain and a cellulose binding module separated by a linker. The genomic DNA of Ha-eng-1a is 2,129-bp long, containing eight introns ranging from 56 bp to157 bp and nine exons ranging from 70 bp to 299 bp. Southern blot analysis revealed that the Ha-eng-1a gene has two copies. In situ hybridization showed that the Ha-eng-1a transcriptsspecifically accumulated in the two subventral gland cells of the second-stage juveniles. There was evidence for cellulase activity of the recombinant protein Ha-eng-1a in vitro. The results indicated that this beta-1,4-endoglucanase gene may play a crucial role in plant cell wall-degradation during penetration and migration of nematodes in the host roots.

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